8VFS

oppF ROSE-like RNA thermometer structure

8VFS の概要

• エントリーDOI: 10.2210/pdb8vfs/pdb
• 分子名称: RNA (27-MER) (2 entities in total)
• 機能のキーワード: rna thermometer, regulatory rna, abc transporters, thermoswitch, rna
• 由来する生物種: Providencia stuartii
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 17364.35

構造登録者

Shaffer, S.N.,Passalacqua, L.F.M.,Abdelsayed, M.M.,Ferre-D'Amare, A.R. (登録日: 2023-12-21, 公開日: 2024-12-25, 最終更新日: 2025-01-22)

主引用文献

Tong, A.Y.,Tong, E.L.,Hannani, M.A.,Shaffer, S.N.,Santiago, D.,Ferre-D'Amare, A.R.,Passalacqua, L.F.M.,Abdelsayed, M.M.
RNA thermometers are widespread upstream of ABC transporter genes in bacteria.
J.Biol.Chem., 300:107547-107547, 2024

PubMed Abstract: RNA thermometers are temperature-sensing non-coding RNAs that regulate the expression of downstream genes. A well-characterized RNA thermometer motif discovered in bacteria is the ROSE-like element (repression of heat shock gene expression). ATP-binding cassette (ABC) transporters are a superfamily of transmembrane proteins that harness ATP hydrolysis to facilitate the export and import of substrates across cellular membranes. Through structure-guided bioinformatics, we discovered that ROSE-like RNA thermometers are widespread upstream of ABC transporter genes in bacteria. X-ray crystallography, biochemistry, and cellular assays indicate that these RNA thermometers are functional regulatory elements. This study expands the known biological role of RNA thermometers to these key membrane transporters.

PubMed: 38992441
DOI: 10.1016/j.jbc.2024.107547

実験手法

X-RAY DIFFRACTION (2.7 Å)