8VD3

Crystal Structure of D14 from Arabidopsis thaliana

8VD3 の概要

• エントリーDOI: 10.2210/pdb8vd3/pdb
• 分子名称: Strigolactone esterase D14 (2 entities in total)
• 機能のキーワード: strigolactone, signalling, alpha/beta-hydrolase, phytohormone, hydrolase
• 由来する生物種: Arabidopsis thaliana (thale cress)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 59307.45

構造登録者

Gilio, A.K.,Shabek, N.,Guercio, A.M.,Pawlak, J. (登録日: 2023-12-14, 公開日: 2024-07-31, 最終更新日: 2024-08-21)

主引用文献

Guercio, A.M.,Gilio, A.K.,Pawlak, J.,Shabek, N.
Structural insights into rice KAI2 receptor provide functional implications for perception and signal transduction.
J.Biol.Chem., 300:107593-107593, 2024

PubMed Abstract: KAI2 receptors, classified as plant α/β hydrolase enzymes, are capable of perceiving smoke-derived butenolide signals and endogenous yet unidentified KAI2-ligands (KLs). While the number of functional KAI2 receptors varies among land plant species, rice has only one KAI2 gene. Rice, a significant crop and representative of grasses, relies on KAI2-mediated Arbuscular mycorrhiza (AM) symbioses to flourish in traditionally arid and nutrient-poor environments. This study presents the first crystal structure of an active rice (Oryza sativa, Os) KAI2 hydrolase receptor. Our structural and biochemical analyses uncover grass-unique pocket residues influencing ligand sensitivity and hydrolytic activity. Through structure-guided analysis, we identify a specific residue whose mutation enables the increase or decrease of ligand perception, catalytic activity, and signal transduction. Furthermore, we investigate OsKAI2-mediated signaling by examining its ability to form a complex with its binding partner, the F-box protein DWARF3 (D3) ubiquitin ligase and subsequent degradation of the target substrate OsSMAX1, demonstrating the significant role of hydrophobic interactions in the OsKAI2-D3 interface. This study provides new insights into the diverse and pivotal roles of the OsKAI2 signaling pathway in the plant kingdom, particularly in grasses.

PubMed: 39032651
DOI: 10.1016/j.jbc.2024.107593

実験手法

X-RAY DIFFRACTION (2 Å)