8V35

Crystal structure of HpsN from Cupriavidus pinatubonensis

8V35 の概要

• エントリーDOI: 10.2210/pdb8v35/pdb
• 分子名称: Sulfopropanediol 3-dehydrogenase, 1,2-ETHANEDIOL, ZINC ION, ... (4 entities in total)
• 機能のキーワード: dhps, dehydrogenase, nad-dependent, sulfolactate, oxidoreductase
• 由来する生物種: Cupriavidus pinatubonensis JMP134
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 94175.23

構造登録者

Lee, M. (登録日: 2023-11-27, 公開日: 2024-09-25, 最終更新日: 2024-10-09)

主引用文献

Burchill, L.,Kaur, A.,Nastasovici, A.,Lee, M.,Williams, S.J.
Structural and kinetic insights into the stereospecific oxidation of R -2,3-dihydroxypropanesulfonate by DHPS-3-dehydrogenase from Cupriavidus pinatubonensis.
Chem Sci, 15:15757-15768, 2024

PubMed Abstract: 2,3-Dihydroxypropanesulfonate (DHPS) and sulfolactate (SL) are environmentally important organosulfur compounds that play key roles as metabolic currencies in the sulfur cycle. Despite their prevalence, the pathways governing DHPS and SL production remain poorly understood. Here, we study DHPS-3-dehydrogenase from (HpsN), a bacterium capable of utilizing DHPS as a sole carbon source. Kinetic analysis of HpsN reveals a strict preference for -DHPS, catalyzing its 4-electron oxidation to -SL, with high specificity for NAD over NADP. The 3D structure of HpsN in complex with Zn, NADH and -SL, elucidated through X-ray crystallography, reveals a fold akin to bacterial and plant histidinol dehydrogenases with similar coordination geometry around the octahedral Zn centre and involving the sulfonate group as a ligand. A key residue, His126, distinguishes DHPS dehydrogenases from histidinol dehydrogenases, by structural recognition of the sulfonate substrate of the former. Site-directed mutagenesis pinpoints Glu318, His319, and Asp352 as active-site residues important for the catalytic activity of HpsN. Taxonomic and pathway distribution analysis reveals the prevalence of HpsN homologues within different pathways of DHPS catabolism and across bacterial classes including Alpha-, Beta-, Gamma-, and Deltaproteobacteria and Desulfobacteria, emphasizing its importance in the biogeochemical sulfur cycle.

PubMed: 39263660
DOI: 10.1039/d4sc05114a

実験手法

X-RAY DIFFRACTION (1.94 Å)