8V26

H2BK120ub-modified nucleosome ubiquitin position 2

8V26 の概要

• エントリーDOI: 10.2210/pdb8v26/pdb
• 関連するPDBエントリー: 8G6G 8G6H 8G6Q 8G6S 8V25 8V27 8V28
• EMDBエントリー: 29767 29769 29778 29781 42898 42899 42899 42900 42901
• 分子名称: Histone H3.2, Histone H4, Histone H2A, ... (7 entities in total)
• 機能のキーワード: nucleosome, ubiquitin, histone, chromatin, dna binding protein, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Xenopus laevis (African clawed frog); 詳細
• タンパク質・核酸の鎖数: 11
• 化学式量合計: 208923.00

構造登録者

Hicks, C.W.,Wolberger, C. (登録日: 2023-11-21, 公開日: 2024-08-28, 最終更新日: 2024-11-13)

主引用文献

Hicks, C.W.,Rahman, S.,Gloor, S.L.,Fields, J.K.,Husby, N.L.,Vaidya, A.,Maier, K.E.,Morgan, M.,Keogh, M.C.,Wolberger, C.
Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.
Nucleic Acids Res., 52:9978-9995, 2024

PubMed Abstract: Monoubiquitination of histones H2B-K120 (H2BK120ub) and H2A-K119 (H2AK119ub) play opposing roles in regulating transcription and chromatin compaction. H2BK120ub is a hallmark of actively transcribed euchromatin, while H2AK119ub is highly enriched in transcriptionally repressed heterochromatin. Whereas H2BK120ub is known to stimulate the binding or activity of various chromatin-modifying enzymes, this post-translational modification (PTM) also interferes with the binding of several proteins to the nucleosome H2A/H2B acidic patch via an unknown mechanism. Here, we report cryoEM structures of an H2BK120ub nucleosome showing that ubiquitin adopts discrete positions that occlude the acidic patch. Molecular dynamics simulations show that ubiquitin remains stably positioned over this nucleosome region. By contrast, our cryoEM structures of H2AK119ub nucleosomes show ubiquitin adopting discrete positions that minimally occlude the acidic patch. Consistent with these observations, H2BK120ub, but not H2AK119ub, abrogates nucleosome interactions with acidic patch-binding proteins RCC1 and LANA, and single-domain antibodies specific to this region. Our results suggest a mechanism by which H2BK120ub serves as a gatekeeper to the acidic patch and point to distinct roles for histone H2AK119 and H2BK120 ubiquitination in regulating protein binding to nucleosomes.

PubMed: 39149911
DOI: 10.1093/nar/gkae698

実験手法

ELECTRON MICROSCOPY (3.33 Å)