8V10

Structure of a Saccharomyces cerevisiae Mps1 peptide bound to dwarf Ndc80 Complex

8V10 の概要

• エントリーDOI: 10.2210/pdb8v10/pdb
• 分子名称: Kinetochore protein NDC80, Mps1/NUF2 chimera protein, Kinetochore protein SPC24, ... (5 entities in total)
• 機能のキーワード: tension sensing, ndc80, kinetochore, cell cycle
• 由来する生物種: Saccharomyces cerevisiae (brewer's yeast); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 85394.91

構造登録者

Zahm, J.A.,Harrison, S.C. (登録日: 2023-11-19, 公開日: 2024-05-15, 最終更新日: 2024-11-20)

主引用文献

Zahm, J.A.,Harrison, S.C.
A communication hub for phosphoregulation of kinetochore-microtubule attachment.
Curr.Biol., 34:2308-2318.e6, 2024

PubMed Abstract: The Mps1 and Aurora B kinases regulate and monitor kinetochore attachment to spindle microtubules during cell division, ultimately ensuring accurate chromosome segregation. In yeast, the critical spindle attachment components are the Ndc80 and Dam1 complexes (Ndc80c and DASH/Dam1c, respectively). Ndc80c is a 600-Å-long heterotetramer that binds microtubules through a globular "head" at one end and centromere-proximal kinetochore components through a globular knob at the other end. Dam1c is a heterodecamer that forms a ring of 16-17 protomers around the shaft of the single kinetochore microtubule in point-centromere yeast. The ring coordinates the approximately eight Ndc80c rods per kinetochore. In published work, we showed that a site on the globular "head" of Ndc80c, including residues from both Ndc80 and Nuf2, binds a bipartite segment in the long C-terminal extension of Dam1. Results reported here show, both by in vitro binding experiments and by crystal structure determination, that the same site binds a conserved segment in the long N-terminal extension of Mps1. It also binds, less tightly, a conserved segment in the N-terminal extension of Ipl1 (yeast Aurora B). Together with results from experiments in yeast cells and from biochemical assays reported in two accompanying papers, the structures and graded affinities identify a communication hub for ensuring uniform bipolar attachment and for signaling anaphase onset.

PubMed: 38776904
DOI: 10.1016/j.cub.2024.04.067

実験手法

X-RAY DIFFRACTION (3.02 Å)