8UY3

Fem1B with FNIP1 and Tom20 fragment

8UY3 の概要

• エントリーDOI: 10.2210/pdb8uy3/pdb
• 分子名称: Protein fem-1 homolog B, Folliculin-interacting protein 1, Mitochondrial import receptor subunit TOM20 homolog, ... (7 entities in total)
• 機能のキーワード: fem1b fnp1 ubiquitin, proteasome, reductive stress response, tom complex electron transport chain mitochondria, protein binding
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 11
• 化学式量合計: 206610.99

構造登録者

Gee, C.L.,Manford, A.G.,McMinimy, R.,Rape, M. (登録日: 2023-11-12, 公開日: 2025-03-19)

主引用文献

McMinimy, R.,Manford, A.G.,Gee, C.L.,Chandrasekhar, S.,Mousa, G.A.,Chuang, J.,Phu, L.,Shih, K.Y.,Rose, C.M.,Kuriyan, J.,Bingol, B.,Rape, M.
Reactive oxygen species control protein degradation at the mitochondrial import gate.
Mol.Cell, 84:4612-4628.e13, 2024

PubMed Abstract: While reactive oxygen species (ROS) have long been known to drive aging and neurodegeneration, their persistent depletion below basal levels also disrupts organismal function. Cells counteract loss of basal ROS via the reductive stress response, but the identity and biochemical activity of ROS sensed by this pathway remain unknown. Here, we show that the central enzyme of the reductive stress response, the E3 ligase Cullin 2-FEM1 homolog B (CUL2), specifically acts at mitochondrial TOM complexes, where it senses ROS produced by complex III of the electron transport chain (ETC). ROS depletion during times of low ETC activity triggers the localized degradation of CUL2 substrates, which sustains mitochondrial import and ensures the biogenesis of the rate-limiting ETC complex IV. As complex III yields most ROS when the ETC outpaces metabolic demands or oxygen availability, basal ROS are sentinels of mitochondrial activity that help cells adjust their ETC to changing environments, as required for cell differentiation and survival.

PubMed: 39642856
DOI: 10.1016/j.molcel.2024.11.004

実験手法

X-RAY DIFFRACTION (3.2 Å)