8UVX

CosR DNA bound form I

Summary for 8UVX

• Entry DOI: 10.2210/pdb8uvx/pdb
• Descriptor: DNA-binding response regulator, DNA (5'-D(*AP*TP*AP*TP*CP*TP*TP*AP*AP*TP*TP*TP*TP*GP*GP*TP*TP*AP*AP*TP*A)-3'), DNA (5'-D(*TP*AP*TP*TP*AP*AP*CP*CP*AP*AP*AP*AP*TP*TP*AP*AP*GP*AP*TP*AP*T)-3'), ... (4 entities in total)
• Functional Keywords: cosr, transcriptional regulator, campylobacter jejuni, dna binding protein, dna binding protein-dna complex, dna binding protein/dna
• Biological source: Campylobacter jejuni; More
• Total number of polymer chains: 4
• Total formula weight: 63886.88

Authors

Zhang, Z. (deposition date: 2023-11-05, release date: 2024-01-31, Last modification date: 2024-04-03)

Primary citation

Zhang, Z.,Yan, Y.,Pang, J.,Dai, L.,Zhang, Q.,Yu, E.W.
Structural basis of DNA recognition of the Campylobacter jejuni CosR regulator.
Mbio, 15:e0343023-e0343023, 2024

PubMed Abstract: is a foodborne pathogen commonly found in the intestinal tracts of animals. This pathogen is a leading cause of gastroenteritis in humans. Besides its highly infectious nature, is increasingly resistant to a number of clinically administrated antibiotics. As a consequence, the Centers for Disease Control and Prevention has designated antibiotic-resistant as a serious antibiotic resistance threat in the United States. The CosR regulator is essential to the viability of this bacterium and is responsible for regulating the expression of a number of oxidative stress defense enzymes. Importantly, it also modulates the expression of the CmeABC multidrug efflux system, the most predominant and clinically important system in that mediates resistance to multiple antimicrobials. Here, we report structures of apo-CosR and CosR bound with a 21 bp DNA sequence located at the promotor region using both single-particle cryo-electron microscopy and X-ray crystallography. These structures allow us to propose a novel mechanism for CosR regulation that involves a long-distance conformational coupling and rearrangement of the secondary structural elements of the regulator to bind target DNA.

PubMed: 38323832
DOI: 10.1128/mbio.03430-23

Experimental method

X-RAY DIFFRACTION (2.9 Å)