8UVX

CosR DNA bound form I

8UVX の概要

• エントリーDOI: 10.2210/pdb8uvx/pdb
• 分子名称: DNA-binding response regulator, DNA (5'-D(*AP*TP*AP*TP*CP*TP*TP*AP*AP*TP*TP*TP*TP*GP*GP*TP*TP*AP*AP*TP*A)-3'), DNA (5'-D(*TP*AP*TP*TP*AP*AP*CP*CP*AP*AP*AP*AP*TP*TP*AP*AP*GP*AP*TP*AP*T)-3'), ... (4 entities in total)
• 機能のキーワード: cosr, transcriptional regulator, campylobacter jejuni, dna binding protein, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Campylobacter jejuni; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 63886.88

構造登録者

Zhang, Z. (登録日: 2023-11-05, 公開日: 2024-01-31, 最終更新日: 2024-04-03)

主引用文献

Zhang, Z.,Yan, Y.,Pang, J.,Dai, L.,Zhang, Q.,Yu, E.W.
Structural basis of DNA recognition of the Campylobacter jejuni CosR regulator.
Mbio, 15:e0343023-e0343023, 2024

PubMed Abstract: is a foodborne pathogen commonly found in the intestinal tracts of animals. This pathogen is a leading cause of gastroenteritis in humans. Besides its highly infectious nature, is increasingly resistant to a number of clinically administrated antibiotics. As a consequence, the Centers for Disease Control and Prevention has designated antibiotic-resistant as a serious antibiotic resistance threat in the United States. The CosR regulator is essential to the viability of this bacterium and is responsible for regulating the expression of a number of oxidative stress defense enzymes. Importantly, it also modulates the expression of the CmeABC multidrug efflux system, the most predominant and clinically important system in that mediates resistance to multiple antimicrobials. Here, we report structures of apo-CosR and CosR bound with a 21 bp DNA sequence located at the promotor region using both single-particle cryo-electron microscopy and X-ray crystallography. These structures allow us to propose a novel mechanism for CosR regulation that involves a long-distance conformational coupling and rearrangement of the secondary structural elements of the regulator to bind target DNA.

PubMed: 38323832
DOI: 10.1128/mbio.03430-23

実験手法

X-RAY DIFFRACTION (2.9 Å)