8UR2

Crystal Structure of macrophage migration inhibitory factor (MIF) from Trichomonas vaginalis (I41 form)

8UR2 の概要

• エントリーDOI: 10.2210/pdb8ur2/pdb
• 分子名称: MACROPHAGE MIGRATION INHIBITORY FACTOR, IODIDE ION, PYRUVIC ACID, ... (4 entities in total)
• 機能のキーワード: ssgcid, structural genomics, seattle structural genomics center for infectious disease, macrophage migration inhibitory factor, cytokine
• 由来する生物種: Trichomonas vaginalis G3
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 89271.75

構造登録者

Seattle Structural Genomics Center for Infectious Disease,Seattle Structural Genomics Center for Infectious Disease (SSGCID) (登録日: 2023-10-25, 公開日: 2023-11-08, 最終更新日: 2024-12-11)

主引用文献

Srivastava, A.,Nair, A.,Dawson, O.C.O.,Gao, R.,Liu, L.,Craig, J.K.,Battaile, K.P.,Harmon, E.K.,Barrett, L.K.,Van Voorhis, W.C.,Subramanian, S.,Myler, P.J.,Lovell, S.,Asojo, O.A.,Darwiche, R.
Structures of Trichomonas vaginalis macrophage migratory inhibitory factor.
Acta Crystallogr.,Sect.F, 80:341-347, 2024

PubMed Abstract: The unicellular parasitic protozoan Trichomonas vaginalis causes trichomoniasis, the most prevalent nonviral sexually transmitted disease globally. T. vaginalis evades host immune responses by producing homologs of host proteins, including cytokines such as macrophage migration inhibitory factor. T. vaginalis macrophage migration inhibitory factor (TvMIF) helps to facilitate the survival of T. vaginalis during nutritional stress conditions, increases prostate cell proliferation and invasiveness, and induces inflammation-related cellular pathways, thus mimicking the ability of human MIF to increase inflammation and cell proliferation. The production, crystallization and three structures of N-terminally hexahistidine-tagged TvMIF reveal a prototypical MIF trimer with a topology similar to that of human homologs (hMIF-1 and hMIF-2). The N-terminal tag obscures the expected pyruvate-binding site. The similarity of TvMIF to its human homologs can be exploited for structure-based drug discovery.

PubMed: 39601418
DOI: 10.1107/S2053230X24011105

実験手法

X-RAY DIFFRACTION (1.9 Å)