8UO6

HIV-1 Rev Response Element (RRE) Stem-Loop II (SLII)

Summary for 8UO6

• Entry DOI: 10.2210/pdb8uo6/pdb
• Descriptor: HIV-1 Rev Response Element Stem-Loop II with tRNA scaffold (1 entity in total)
• Functional Keywords: non-coding rna, hiv, nuclear export, rre, viral rna, rna
• Biological source: HIV whole-genome vector AA1305#18
• Total number of polymer chains: 2
• Total formula weight: 86683.39

Authors

Tipo, J.,Gottipati, K.,Choi, K. (deposition date: 2023-10-19, release date: 2024-06-05)

Primary citation

Tipo, J.,Gottipati, K.,Slaton, M.,Gonzalez-Gutierrez, G.,Choi, K.H.
Structure of HIV-1 RRE stem-loop II identifies two conformational states of the high-affinity Rev binding site.
Nat Commun, 15:4198-4198, 2024

PubMed Abstract: During HIV infection, specific RNA-protein interaction between the Rev response element (RRE) and viral Rev protein is required for nuclear export of intron-containing viral mRNA transcripts. Rev initially binds the high-affinity site in stem-loop II, which promotes oligomerization of additional Rev proteins on RRE. Here, we present the crystal structure of RRE stem-loop II in distinct closed and open conformations. The high-affinity Rev-binding site is located within the three-way junction rather than the predicted stem IIB. The closed and open conformers differ in their non-canonical interactions within the three-way junction, and only the open conformation has the widened major groove conducive to initial Rev interaction. Rev binding assays show that RRE stem-loop II has high- and low-affinity binding sites, each of which binds a Rev dimer. We propose a binding model, wherein Rev-binding sites on RRE are sequentially created through structural rearrangements induced by Rev-RRE interactions.

PubMed: 38760344
DOI: 10.1038/s41467-024-48162-y

Experimental method

X-RAY DIFFRACTION (2.85 Å)