8UGP

In-situ structure of typeA supercomplex in respiratory chain ( local refined map focused on CI iron-sulfur cluster regions )

これはPDB形式変換不可エントリーです。

8UGP の概要

• エントリーDOI: 10.2210/pdb8ugp/pdb
• EMDBエントリー: 42231
• 分子名称: NADH-ubiquinone oxidoreductase chain 3, NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6, ... (14 entities in total)
• 機能のキーワード: in-situ cryo-em structure, mammalian, mitochondria, respiratory supercomplex, proton pumping, membrane protein, electron transport
• 由来する生物種: Sus scrofa (pig); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 377112.76

構造登録者

Zheng, W.,Zhang, K.,Zhu, J. (登録日: 2023-10-05, 公開日: 2024-06-19, 最終更新日: 2024-07-17)

主引用文献

Zheng, W.,Chai, P.,Zhu, J.,Zhang, K.
High-resolution in situ structures of mammalian respiratory supercomplexes.
Nature, 631:232-239, 2024

PubMed Abstract: Mitochondria play a pivotal part in ATP energy production through oxidative phosphorylation, which occurs within the inner membrane through a series of respiratory complexes. Despite extensive in vitro structural studies, determining the atomic details of their molecular mechanisms in physiological states remains a major challenge, primarily because of loss of the native environment during purification. Here we directly image porcine mitochondria using an in situ cryo-electron microscopy approach. This enables us to determine the structures of various high-order assemblies of respiratory supercomplexes in their native states. We identify four main supercomplex organizations: IIIIIV, IIIIIV, IIIIIV and IIIIIV, which potentially expand into higher-order arrays on the inner membranes. These diverse supercomplexes are largely formed by 'protein-lipids-protein' interactions, which in turn have a substantial impact on the local geometry of the surrounding membranes. Our in situ structures also capture numerous reactive intermediates within these respiratory supercomplexes, shedding light on the dynamic processes of the ubiquinone/ubiquinol exchange mechanism in complex I and the Q-cycle in complex III. Structural comparison of supercomplexes from mitochondria treated under different conditions indicates a possible correlation between conformational states of complexes I and III, probably in response to environmental changes. By preserving the native membrane environment, our approach enables structural studies of mitochondrial respiratory supercomplexes in reaction at high resolution across multiple scales, from atomic-level details to the broader subcellular context.

PubMed: 38811722
DOI: 10.1038/s41586-024-07488-9

実験手法

ELECTRON MICROSCOPY (2 Å)