8UDN

A Stable Heterotrimeric Foldon with Minimum Mutation

8UDN の概要

• エントリーDOI: 10.2210/pdb8udn/pdb
• 分子名称: Fibritin (2 entities in total)
• 機能のキーワード: foldon heterotrimer self-assembly peptides miniprotein, viral protein
• 由来する生物種: Tequatrovirus T4
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 9388.56

構造登録者

Bingman, C.A.,Liu, X.,Roberts, D.S.,Gellman, S.H. (登録日: 2023-09-28, 公開日: 2025-05-14, 最終更新日: 2025-07-23)

主引用文献

Liu, X.,Roberts, D.S.,Bingman, C.A.,Ge, Y.,Gellman, S.H.
Rational Design of a Foldon-Derived Heterotrimer Guided by Quantitative Native Mass Spectrometry.
J.Am.Chem.Soc., 147:23819-23826, 2025

PubMed Abstract: Designing stable hetero-oligomeric protein complexes with defined intersubunit stoichiometry remains a significant challenge. In this study, we report the design of a highly selective heterotrimeric assembly derived from the well-known foldon homotrimer. We generated an heterotrimer by replacing glutamine 11 with glutamic acid, to destabilize the homotrimer, and replacing valine 14 with either alanine or leucine, to stabilize the hydrophobic core of the heterotrimer. Native mass spectrometry (MS) was essential for guiding the design process, enabling precise characterization of oligomeric states and their equilibrium distributions. The heterotrimer structure was validated by X-ray crystallography. Our findings highlight the effectiveness of combining rational design with native MS to develop specific hetero-oligomeric assemblies.

PubMed: 40580137
DOI: 10.1021/jacs.5c06094

実験手法

X-RAY DIFFRACTION (0.97 Å)