8U3Y

SpG Cas9 with NGG PAM DNA target

Summary for 8U3Y

• Entry DOI: 10.2210/pdb8u3y/pdb
• EMDB information: 41867
• Descriptor: CRISPR-associated endonuclease Cas9/Csn1, RNA (98-MER), DNA (5'-D(P*CP*GP*TP*TP*TP*GP*TP*AP*CP*TP*CP*CP*AP*GP*CP*G)-3'), ... (7 entities in total)
• Functional Keywords: spg-cas9, crispr, cas9, immune system, r-loop, immune system-dna-rna complex, immune system/dna/rna
• Biological source: Streptococcus pyogenes; More
• Total number of polymer chains: 5
• Total formula weight: 204310.33

Authors

Bravo, J.P.K.,Hibshman, G.N.,Taylor, D.W. (deposition date: 2023-09-08, release date: 2024-05-01, Last modification date: 2024-05-15)

• Primary citation: Hibshman, G.N.,Bravo, J.P.K.,Hooper, M.M.,Dangerfield, T.L.,Zhang, H.,Finkelstein, I.J.,Johnson, K.A.,Taylor, D.W.; Unraveling the mechanisms of PAMless DNA interrogation by SpRY-Cas9.; Nat Commun, 15:3663-3663, 2024; PubMed: 38688943; DOI: 10.1038/s41467-024-47830-3

Experimental method

ELECTRON MICROSCOPY (3.1 Å)