8U26

Gaussian Mixture Models based single particle refinement - GPCR (Substance P bound to active human neurokinin 1 receptor in complex with miniGs399 from EMPIAR-10786)

8U26 の概要

• エントリーDOI: 10.2210/pdb8u26/pdb
• EMDBエントリー: 24570 41840
• 分子名称: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, ... (6 entities in total)
• 機能のキーワード: gpcr, membrane protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 141549.56

構造登録者

Chen, M.,Pintilie, G. (登録日: 2023-09-05, 公開日: 2023-11-15, 最終更新日: 2024-10-09)

主引用文献

Chen, M.,Schmid, M.F.,Chiu, W.
Improving resolution and resolvability of single-particle cryoEM structures using Gaussian mixture models.
Nat.Methods, 21:37-40, 2024

PubMed Abstract: Cryogenic electron microscopy is widely used in structural biology, but its resolution is often limited by the dynamics of the macromolecule. Here we developed a refinement protocol based on Gaussian mixture models that integrates particle orientation and conformation estimation and improves the alignment for flexible domains of protein structures. We demonstrated this protocol on multiple datasets, resulting in improved resolution and resolvability, locally and globally, by visual and quantitative measures.

PubMed: 37973972
DOI: 10.1038/s41592-023-02082-9

実験手法

ELECTRON MICROSCOPY (2.5 Å)