8TWD

Structure of IraM-bound RssB

8TWD の概要

• エントリーDOI: 10.2210/pdb8twd/pdb
• 分子名称: Anti-adapter protein IraM, Regulator of RpoS, ACETATE ION (3 entities in total)
• 機能のキーワード: response regulator, clpxp anti-adaptor, signaling protein
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 93203.12

構造登録者

Brugger, C.,Deaconescu, A.M. (登録日: 2023-08-20, 公開日: 2024-01-10, 最終更新日: 2024-02-14)

主引用文献

Brugger, C.,Srirangam, S.,Deaconescu, A.M.
IraM remodels the RssB segmented helical linker to stabilize sigma s against degradation by ClpXP.
J.Biol.Chem., 300:105568-105568, 2023

PubMed Abstract: Upon Mg starvation, a condition often associated with virulence, enterobacteria inhibit the ClpXP-dependent proteolysis of the master transcriptional regulator, σ, via IraM, a poorly understood antiadaptor that prevents RssB-dependent loading of σ onto ClpXP. This inhibition results in σ accumulation and expression of stress resistance genes. Here, we report on the structural analysis of RssB bound to IraM, which reveals that IraM induces two folding transitions within RssB, amplified via a segmented helical linker. These conformational changes result in an open, yet inhibited RssB structure in which IraM associates with both the C-terminal and N-terminal domains of RssB and prevents binding of σ to the 4-5-5 face of the N-terminal receiver domain. This work highlights the remarkable structural plasticity of RssB and reveals how a stress-specific RssB antagonist modulates a core stress response pathway that could be leveraged to control biofilm formation, virulence, and the development of antibiotic resistance.

PubMed: 38103640
DOI: 10.1016/j.jbc.2023.105568

実験手法

X-RAY DIFFRACTION (3.3 Å)