8TWB

Cryo-EM structure of S. cerevisiae Ctf18-RFC-PCNA-DNA complex

8TWB の概要

• エントリーDOI: 10.2210/pdb8twb/pdb
• EMDBエントリー: 41665
• 分子名称: Replication factor C subunit 4, MAGNESIUM ION, ADENOSINE-5'-DIPHOSPHATE, ... (11 entities in total)
• 機能のキーワード: ctf18, rfc2-5, pcna, dna, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Saccharomyces cerevisiae (brewer's yeast); 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 280663.09

構造登録者

Yuan, Z.,Georgescu, R.,O'Donnell, M.,Li, H. (登録日: 2023-08-20, 公開日: 2024-09-04, 最終更新日: 2024-10-09)

主引用文献

Yuan, Z.,Georgescu, R.,Yao, N.Y.,Yurieva, O.,O'Donnell, M.E.,Li, H.
Mechanism of PCNA loading by Ctf18-RFC for leading-strand DNA synthesis.
Science, 385:eadk5901-eadk5901, 2024

PubMed Abstract: The proliferating cell nuclear antigen (PCNA) clamp encircles DNA to hold DNA polymerases (Pols) to DNA for processivity. The Ctf18-RFC PCNA loader, a replication factor C (RFC) variant, is specific to the leading-strand Pol (Polε). We reveal here the underlying mechanism of Ctf18-RFC specificity to Polε using cryo-electron microscopy and biochemical studies. We found that both Ctf18-RFC and Polε contain specific structural features that direct PCNA loading onto DNA. Unlike other clamp loaders, Ctf18-RFC has a disordered ATPase associated with a diverse cellular activities (AAA+) motor that requires Polε to bind and stabilize it for efficient PCNA loading. In addition, Ctf18-RFC can pry prebound Polε off of DNA, then load PCNA onto DNA and transfer the PCNA-DNA back to Polε. These elements in both Ctf18-RFC and Polε provide specificity in loading PCNA onto DNA for Polε.

PubMed: 39088616
DOI: 10.1126/science.adk5901

実験手法

ELECTRON MICROSCOPY (3.2 Å)