8TQE

XptA2 wild type

これはPDB形式変換不可エントリーです。

8TQE の概要

• エントリーDOI: 10.2210/pdb8tqe/pdb
• EMDBエントリー: 41503
• 分子名称: XptA2 (1 entity in total)
• 機能のキーワード: tca, insecticide, translocase, toxin
• 由来する生物種: Xenorhabdus nematophila
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 1421960.94

構造登録者

Martin, C.L.,Binshtein, E.M.,Aller, S.G. (登録日: 2023-08-07, 公開日: 2023-09-27, 最終更新日: 2023-10-11)

主引用文献

Martin, C.L.,Chester, D.W.,Radka, C.D.,Pan, L.,Yang, Z.,Hart, R.C.,Binshtein, E.M.,Wang, Z.,Nagy, L.,DeLucas, L.J.,Aller, S.G.
Structures of the Insecticidal Toxin Complex Subunit XptA2 Highlight Roles for Flexible Domains.
Int J Mol Sci, 24:-, 2023

PubMed Abstract: The Toxin Complex (Tc) superfamily consists of toxin translocases that contribute to the targeting, delivery, and cytotoxicity of certain pathogenic Gram-negative bacteria. Membrane receptor targeting is driven by the A-subunit (TcA), which comprises IgG-like receptor binding domains (RBDs) at the surface. To better understand XptA2, an insect specific TcA secreted by the symbiont from the intestine of entomopathogenic nematodes, we determined structures by X-ray crystallography and cryo-EM. Contrary to a previous report, XptA2 is pentameric. RBD-B exhibits an indentation from crystal packing that indicates loose association with the shell and a hotspot for possible receptor binding or a trigger for conformational dynamics. A two-fragment XptA2 lacking an intact linker achieved the folded pre-pore state like wild type (wt), revealing no requirement of the linker for protein folding. The linker is disordered in all structures, and we propose it plays a role in dynamics downstream of the initial pre-pore state.

PubMed: 37686027
DOI: 10.3390/ijms241713221

実験手法

ELECTRON MICROSCOPY (3.1 Å)