8TO0

48-nm repeating structure of doublets from mouse sperm flagella

これはPDB形式変換不可エントリーです。

8TO0 の概要

• エントリーDOI: 10.2210/pdb8to0/pdb
• EMDBエントリー: 41315 41316 41431
• 分子名称: Cilia- and flagella-associated protein 95, Cilia- and flagella-associated protein 53, Tektin bundle-interacting protein 1, ... (35 entities in total)
• 機能のキーワード: mammalian sperm, axoneme, microtubule-based structure, microtubule inner protein, non-motor proteins, cellular motility, fertility, structural protein
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 471
• 化学式量合計: 22692158.01

構造登録者

Chen, Z.,Shiozak, M.,Hass, K.M.,Skinner, W.,Zhao, S.,Guo, C.,Polacco, B.J.,Yu, Z.,Krogan, N.J.,Kaake, R.M.,Vale, R.D.,Agard, D.A. (登録日: 2023-08-02, 公開日: 2023-11-01, 最終更新日: 2023-11-22)

主引用文献

Chen, Z.,Shiozaki, M.,Haas, K.M.,Skinner, W.M.,Zhao, S.,Guo, C.,Polacco, B.J.,Yu, Z.,Krogan, N.J.,Lishko, P.V.,Kaake, R.M.,Vale, R.D.,Agard, D.A.
De novo protein identification in mammalian sperm using in situ cryoelectron tomography and AlphaFold2 docking.
Cell, 186:5041-5053.e19, 2023

PubMed Abstract: To understand the molecular mechanisms of cellular pathways, contemporary workflows typically require multiple techniques to identify proteins, track their localization, and determine their structures in vitro. Here, we combined cellular cryoelectron tomography (cryo-ET) and AlphaFold2 modeling to address these questions and understand how mammalian sperm are built in situ. Our cellular cryo-ET and subtomogram averaging provided 6.0-Å reconstructions of axonemal microtubule structures. The well-resolved tertiary structures allowed us to unbiasedly match sperm-specific densities with 21,615 AlphaFold2-predicted protein models of the mouse proteome. We identified Tektin 5, CCDC105, and SPACA9 as novel microtubule-associated proteins. These proteins form an extensive interaction network crosslinking the lumen of axonemal doublet microtubules, suggesting their roles in modulating the mechanical properties of the filaments. Indeed, Tekt5 -/- sperm possess more deformed flagella with 180° bends. Together, our studies presented a cellular visual proteomics workflow and shed light on the in vivo functions of Tektin 5.

PubMed: 37865089
DOI: 10.1016/j.cell.2023.09.017

実験手法

ELECTRON MICROSCOPY (7.7 Å)