8TN8

Crystal structure of the murine astrovirus capsid spike at 1.75 A

8TN8 の概要

• エントリーDOI: 10.2210/pdb8tn8/pdb
• 分子名称: Capsid polyprotein VP90, POTASSIUM ION (3 entities in total)
• 機能のキーワード: capsid, spike, attachment, homodimer, viral protein
• 由来する生物種: Murine astrovirus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 58593.06

構造登録者

Lanning, S.,DuBois, R.M. (登録日: 2023-08-01, 公開日: 2023-11-15)

主引用文献

Lanning, S.,Pedicino, N.,Haley, D.J.,Hernandez, S.,Cortez, V.,DuBois, R.M.
Structure and immunogenicity of the murine astrovirus capsid spike.
J.Gen.Virol., 104:-, 2023

PubMed Abstract: Human astroviruses (HAstVs) are small, non-enveloped icosahedral RNA viruses that are a significant cause of diarrhoea in young children. Despite their worldwide prevalence, HAstV pathogenesis studies and vaccine development remain challenging due to the lack of an animal model for HAstV infection. The recent development of a murine astrovirus (MuAstV) infection model in mice provides the opportunity to test proof-of-concept vaccines based on MuAstV antigens. To help establish a system in which an astrovirus capsid spike-based vaccine could be tested , we designed and produced a recombinant MuAstV capsid spike protein based on predicted secondary structure homology to HAstV spike proteins. The recombinant MuAstV spike can be expressed with high efficiency in and retains antigenicity to polyclonal antibodies elicited by MuAstV infection. We determined the crystal structure of the MuAstV spike to 1.75 Å and assessed its structural conservation with HAstV capsid spike. Despite low sequence identity between the MuAstV and HAstV spikes and differences in their overall shapes, they share related structural folds. Additionally, we found that vaccination with MuAstV spike induced anti-MuAstV-spike antibodies, highlighting that the recombinant spike is immunogenic. These studies lay a foundation for future MuAstV challenge studies to test whether MuAstV spike can be the basis of an effective vaccine.

PubMed: 37910165
DOI: 10.1099/jgv.0.001913

実験手法

X-RAY DIFFRACTION (1.75 Å)