8TKQ

Cryo-EM structure of human full-length RAD52

8TKQ の概要

• エントリーDOI: 10.2210/pdb8tkq/pdb
• EMDBエントリー: 41357
• 分子名称: DNA repair protein RAD52 homolog (1 entity in total)
• 機能のキーワード: dna repair, recombination
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 11
• 化学式量合計: 532444.22

構造登録者

Schnicker, N.J.,Razzaghi, M.,Spies, M. (登録日: 2023-07-25, 公開日: 2024-08-14, 最終更新日: 2025-05-14)

主引用文献

Honda, M.,Razzaghi, M.,Gaur, P.,Malacaria, E.,Marozzi, G.,Di Biagi, L.,Aiello, F.A.,Paintsil, E.A.,Stanfield, A.J.,Deppe, B.J.,Gakhar, L.,Schnicker, N.J.,Spies, M.A.,Pichierri, P.,Spies, M.
The RAD52 double-ring remodels replication forks restricting fork reversal.
Nature, 641:512-519, 2025

PubMed Abstract: Human RAD52 is a multifunctional DNA repair protein involved in several cellular events that support genome stability, including protection of stalled DNA replication forks from excessive degradation. In its gatekeeper role, RAD52 binds to and stabilizes stalled replication forks during replication stress, protecting them from reversal by SMARCAL1 motor. The structural and molecular mechanism of the RAD52-mediated fork protection remains elusive. Here, using P1 nuclease sensitivity, biochemical and single-molecule analyses, we show that RAD52 dynamically remodels replication forks through its strand exchange activity. The presence of the single-stranded DNA binding protein RPA at the fork modulates the kinetics of the strand exchange without impeding the reaction outcome. Mass photometry and single-particle cryo-electron microscopy show that the replication fork promotes a unique nucleoprotein structure containing head-to-head arrangement of two undecameric RAD52 rings with an extended positively charged surface that accommodates all three arms of the replication fork. We propose that the formation and continuity of this surface is important for the strand exchange reaction and for competition with SMARCAL1.

PubMed: 40175552
DOI: 10.1038/s41586-025-08753-1

実験手法

ELECTRON MICROSCOPY (2.5 Å)