8TIE

Double nuclear outer ring of Nup84-complexes from the yeast NPC

8TIE の概要

• エントリーDOI: 10.2210/pdb8tie/pdb
• 関連するPDBエントリー: 8T9L 8TJ5
• EMDBエントリー: 41114 41116 41117 41119 41120 41121 41122 41123 41285 41300
• 分子名称: Nucleoporin NUP120, Nucleoporin NUP85, NUP145 isoform 1, ... (7 entities in total)
• 機能のキーワード: nuclear pore complex, nucleocytoplasmic transport, nucleoporin, membrane protein, translocase, transport protein
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 1272499.85

構造登録者

Akey, C.W.,Echeverria, I.,Ouch, C.,Fernandez-Martinez, J.,Rout, M.P. (登録日: 2023-07-19, 公開日: 2023-10-11)

主引用文献

Akey, C.W.,Echeverria, I.,Ouch, C.,Nudelman, I.,Shi, Y.,Wang, J.,Chait, B.T.,Sali, A.,Fernandez-Martinez, J.,Rout, M.P.
Implications of a multiscale structure of the yeast nuclear pore complex.
Mol.Cell, 83:3283-3302.e5, 2023

PubMed Abstract: Nuclear pore complexes (NPCs) direct the nucleocytoplasmic transport of macromolecules. Here, we provide a composite multiscale structure of the yeast NPC, based on improved 3D density maps from cryogenic electron microscopy and AlphaFold2 models. Key features of the inner and outer rings were integrated into a comprehensive model. We resolved flexible connectors that tie together the core scaffold, along with equatorial transmembrane complexes and a lumenal ring that anchor this channel within the pore membrane. The organization of the nuclear double outer ring reveals an architecture that may be shared with ancestral NPCs. Additional connections between the core scaffold and the central transporter suggest that under certain conditions, a degree of local organization is present at the periphery of the transport machinery. These connectors may couple conformational changes in the scaffold to the central transporter to modulate transport. Collectively, this analysis provides insights into assembly, transport, and NPC evolution.

PubMed: 37738963
DOI: 10.1016/j.molcel.2023.08.025

実験手法

ELECTRON MICROSCOPY (8.1 Å)