8TEM

Cryo-EM structure of Arabidopsis thaliana Bor1 mutant (R637E/E641R/R643E) in the inward-facing conformation in lauryl maltose neopentyl glycol (LMNG)

8TEM の概要

• エントリーDOI: 10.2210/pdb8tem/pdb
• EMDBエントリー: 41185 41186 41187 41188 41190 41191 41192
• 分子名称: Boron transporter 1 (1 entity in total)
• 機能のキーワード: borate transporter, bor1, transport protein
• 由来する生物種: Arabidopsis thaliana (thale cress)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 160217.80

構造登録者

Jiang, Y.,Jiang, J. (登録日: 2023-07-06, 公開日: 2024-07-10, 最終更新日: 2025-01-22)

主引用文献

Jiang, Y.,Jiang, J.
The Bor1 elevator transport cycle is subject to autoinhibition and activation.
Nat Commun, 15:9090-9090, 2024

PubMed Abstract: Boron, essential for plant growth, necessitates precise regulation due to its potential toxicity. This regulation is achieved by borate transporters (BORs), which are homologous to the SLC4 family. The Arabidopsis thaliana Bor1 (AtBor1) transporter from clade I undergoes slow regulation through degradation and translational suppression, but its potential for fast regulation via direct activity modulation was unclear. Here, we combine cryo-electron microscopy, mutagenesis, and functional characterization to study AtBor1, revealing high-resolution structures of the dimer in one inactive and three active states. Our findings show that AtBor1 is regulated by two distinct mechanisms: an autoinhibitory domain at the carboxyl terminus obstructs the substrate pathway via conserved salt bridges, and phosphorylation of Thr410 allows interaction with a positively charged pocket at the cytosolic face, essential for borate transport. These results elucidate the molecular basis of AtBor1's activity regulation and highlight its role in fast boron level regulation in plants.

PubMed: 39433547
DOI: 10.1038/s41467-024-53411-1

実験手法

ELECTRON MICROSCOPY (3.02 Å)