8T6O
SpRY-Cas9:gRNA complex targeting TAC PAM DNA with 0 bp R-loop
Summary for 8T6O
Entry DOI | 10.2210/pdb8t6o/pdb |
EMDB information | 41073 |
Descriptor | NTS, CRISPR-associated endonuclease Cas9/Csn1, gRNA, ... (4 entities in total) |
Functional Keywords | spry-cas9, crispr, cas9, immune system, r-loop |
Biological source | Streptococcus pyogenes More |
Total number of polymer chains | 4 |
Total formula weight | 221020.75 |
Authors | Hibshman, G.N.,Bravo, J.P.K.,Taylor, D.W. (deposition date: 2023-06-16, release date: 2024-05-01, Last modification date: 2024-05-15) |
Primary citation | Hibshman, G.N.,Bravo, J.P.K.,Hooper, M.M.,Dangerfield, T.L.,Zhang, H.,Finkelstein, I.J.,Johnson, K.A.,Taylor, D.W. Unraveling the mechanisms of PAMless DNA interrogation by SpRY-Cas9. Nat Commun, 15:3663-3663, 2024 Cited by PubMed: 38688943DOI: 10.1038/s41467-024-47830-3 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (3.1 Å) |
Structure validation
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