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8SZP

Human DHX9 bound to ADP

8SZP の概要
エントリーDOI10.2210/pdb8szp/pdb
分子名称ATP-dependent RNA helicase A, ADENOSINE-5'-DIPHOSPHATE, MAGNESIUM ION, ... (6 entities in total)
機能のキーワードdexh-box, rha, hydrolase
由来する生物種Homo sapiens (human)
タンパク質・核酸の鎖数2
化学式量合計231514.91
構造登録者
Lee, Y.-T.,Sickmier, E.A.,Grigoriu, S.,Boriack-Sjodin, P.A. (登録日: 2023-05-30, 公開日: 2023-08-02, 最終更新日: 2023-11-08)
主引用文献Lee, Y.T.,Sickmier, E.A.,Grigoriu, S.,Castro, J.,Boriack-Sjodin, P.A.
Crystal structures of the DExH-box RNA helicase DHX9.
Acta Crystallogr D Struct Biol, 79:980-991, 2023
Cited by
PubMed Abstract: DHX9 is a DExH-box RNA helicase with versatile functions in transcription, translation, RNA processing and regulation of DNA replication. DHX9 has recently emerged as a promising target for oncology, but to date no mammalian structures have been published. Here, crystal structures of human, dog and cat DHX9 bound to ADP are reported. The three mammalian DHX9 structures share identical structural folds. Additionally, the overall architecture and the individual domain structures of DHX9 are highly conserved with those of MLE, the Drosophila orthologue of DHX9 previously solved in complex with RNA and a transition-state analogue of ATP. Due to differences in the bound substrates and global domain orientations, the localized loop conformations and occupancy of dsRNA-binding domain 2 (dsRBD2) differ between the mammalian DHX9 and MLE structures. The combined effects of the structural changes considerably alter the RNA-binding channel, providing an opportunity to compare active and inactive states of the helicase. Finally, the mammalian DHX9 structures provide a potential tool for structure-based drug-design efforts.
PubMed: 37860960
DOI: 10.1107/S2059798323007611
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2.62 Å)
構造検証レポート
Validation report summary of 8szp
検証レポート(詳細版)ダウンロードをダウンロード

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件を2025-07-23に公開中

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