8SZP

Human DHX9 bound to ADP

8SZP の概要

• エントリーDOI: 10.2210/pdb8szp/pdb
• 分子名称: ATP-dependent RNA helicase A, ADENOSINE-5'-DIPHOSPHATE, MAGNESIUM ION, ... (6 entities in total)
• 機能のキーワード: dexh-box, rha, hydrolase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 231514.91

構造登録者

Lee, Y.-T.,Sickmier, E.A.,Grigoriu, S.,Boriack-Sjodin, P.A. (登録日: 2023-05-30, 公開日: 2023-08-02, 最終更新日: 2023-11-08)

主引用文献

Lee, Y.T.,Sickmier, E.A.,Grigoriu, S.,Castro, J.,Boriack-Sjodin, P.A.
Crystal structures of the DExH-box RNA helicase DHX9.
Acta Crystallogr D Struct Biol, 79:980-991, 2023

PubMed Abstract: DHX9 is a DExH-box RNA helicase with versatile functions in transcription, translation, RNA processing and regulation of DNA replication. DHX9 has recently emerged as a promising target for oncology, but to date no mammalian structures have been published. Here, crystal structures of human, dog and cat DHX9 bound to ADP are reported. The three mammalian DHX9 structures share identical structural folds. Additionally, the overall architecture and the individual domain structures of DHX9 are highly conserved with those of MLE, the Drosophila orthologue of DHX9 previously solved in complex with RNA and a transition-state analogue of ATP. Due to differences in the bound substrates and global domain orientations, the localized loop conformations and occupancy of dsRNA-binding domain 2 (dsRBD2) differ between the mammalian DHX9 and MLE structures. The combined effects of the structural changes considerably alter the RNA-binding channel, providing an opportunity to compare active and inactive states of the helicase. Finally, the mammalian DHX9 structures provide a potential tool for structure-based drug-design efforts.

PubMed: 37860960
DOI: 10.1107/S2059798323007611

実験手法

X-RAY DIFFRACTION (2.62 Å)