8SXJ8SXJ の概要
| エントリーDOI | 10.2210/pdb8sxj/pdb |
| EMDBエントリー | 40854 |
| 分子名称 | Envelope glycoprotein gp160, HIV-1 gp41, CH235.12 Heavy Chain, ... (4 entities in total) |
| 機能のキーワード | hiv-1, envelope, env, ectodomain, fusion protein, viral protein, viral protein-immune system complex, viral protein/immune system |
| 由来する生物種 | Human immunodeficiency virus 1 詳細 |
| タンパク質・核酸の鎖数 | 12 |
| 化学式量合計 | 272991.38 |
| 構造登録者 | |
| 主引用文献 | Bennett, A.L.,Edwards, R.,Kosheleva, I.,Saunders, C.,Bililign, Y.,Williams, A.,Bubphamala, P.,Manosouri, K.,Anasti, K.,Saunders, K.O.,Alam, S.M.,Haynes, B.F.,Acharya, P.,Henderson, R. Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv, 10:eadj0396-eadj0396, 2024 Cited by PubMed Abstract: The HIV-1 Envelope (Env) glycoprotein facilitates host cell fusion through a complex series of receptor-induced structural changes. Although remarkable progress has been made in understanding the structures of various Env conformations, microsecond timescale dynamics have not been studied experimentally. Here, we used time-resolved, temperature-jump small-angle x-ray scattering to monitor structural rearrangements in an HIV-1 Env SOSIP ectodomain construct with microsecond precision. In two distinct Env variants, we detected a transition that correlated with known Env structure rearrangements with a time constant in the hundreds of microseconds range. A previously unknown structural transition was also observed, which occurred with a time constant below 10 μs, and involved an order-to-disorder transition in the trimer apex. Using this information, we engineered an Env SOSIP construct that locks the trimer in the prefusion closed state by connecting adjacent protomers via disulfides. Our findings show that the microsecond timescale structural dynamics play an essential role in controlling the Env conformation with impacts on vaccine design. PubMed: 38306419DOI: 10.1126/sciadv.adj0396 主引用文献が同じPDBエントリー |
| 実験手法 | ELECTRON MICROSCOPY (4 Å) |
構造検証レポート
検証レポート(詳細版)
をダウンロード
をダウンロード
