8SW1

Puromycin-sensitive aminopeptidase with bound peptide

8SW1 の概要

• エントリーDOI: 10.2210/pdb8sw1/pdb
• 分子名称: Puromycin-sensitive aminopeptidase, Polyglutamine peptide, ZINC ION (3 entities in total)
• 機能のキーワード: aminopeptidase, zinc metallopeptidase, m1 family, four domains, polyglutamine peptide, hydrolase
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 103561.17

構造登録者

Rodgers, D.W.,Madabushi, S. (登録日: 2023-05-17, 公開日: 2023-07-26, 最終更新日: 2024-05-22)

主引用文献

Madabushi, S.,Chow, K.M.,Song, E.S.,Goswami, A.,Hersh, L.B.,Rodgers, D.W.
Structure of puromycin-sensitive aminopeptidase and polyglutamine binding.
Plos One, 18:e0287086-e0287086, 2023

PubMed Abstract: Puromycin-sensitive aminopeptidase (E.C. 3.4.11.14, UniProt P55786), a zinc metallopeptidase belonging to the M1 family, degrades a number of bioactive peptides as well as peptides released from the proteasome, including polyglutamine. We report the crystal structure of PSA at 2.3 Ǻ. Overall, the enzyme adopts a V-shaped architecture with four domains characteristic of the M1 family aminopeptidases, but it is in a less compact conformation compared to most M1 enzymes of known structure. A microtubule binding sequence is present in a C-terminal HEAT repeat domain of the enzyme in a position where it might serve to mediate interaction with tubulin. In the catalytic metallopeptidase domain, an elongated active site groove lined with aromatic and hydrophobic residues and a large S1 subsite may play a role in broad substrate recognition. The structure with bound polyglutamine shows a possible interacting mode of this peptide, which is supported by mutation.

PubMed: 37440518
DOI: 10.1371/journal.pone.0287086

実験手法

X-RAY DIFFRACTION (3.65 Å)