8SVC
Crystal Structure of SBP from Klebsiella pneumoniae
Summary for 8SVC
| Entry DOI | 10.2210/pdb8svc/pdb |
| Descriptor | Metal ABC transporter substrate-binding protein, SULFATE ION, ZINC ION, ... (4 entities in total) |
| Functional Keywords | periplasmic solute-binding protein, metal binding protein |
| Biological source | Klebsiella pneumoniae |
| Total number of polymer chains | 1 |
| Total formula weight | 34228.94 |
| Authors | Giles, M.W.,Cole, G.B.,Ng, D.,McDevitt, C.A.,Moraes, T.F. (deposition date: 2023-05-16, release date: 2024-01-24, Last modification date: 2024-05-01) |
| Primary citation | Maunders, E.A.,Giles, M.W.,Ganio, K.,Cunningham, B.A.,Bennett-Wood, V.,Cole, G.B.,Ng, D.,Lai, C.C.,Neville, S.L.,Moraes, T.F.,McDevitt, C.A.,Tan, A. Zinc acquisition and its contribution to Klebsiella pneumoniae virulence. Front Cell Infect Microbiol, 13:1322973-1322973, 2023 Cited by PubMed Abstract: is a World Health Organization priority pathogen and a significant clinical concern for infections of the respiratory and urinary tracts due to widespread and increasing resistance to antimicrobials. In the absence of a vaccine, there is an urgent need to identify novel targets for therapeutic development. Bacterial pathogens, including , require the -block metal ion zinc as an essential micronutrient, which serves as a cofactor for ~6% of the proteome. During infection, zinc acquisition necessitates the use of high affinity uptake systems to overcome niche-specific zinc limitation and host-mediated nutritional immunity. Here, we report the identification of ZnuCBA and ZniCBA, two ATP-binding cassette permeases that are highly conserved in species and contribute to AJ218 zinc homeostasis, and the high-resolution structure of the zinc-recruiting solute-binding protein ZniA. The Znu and Zni permeases appear functionally redundant with abrogation of both systems required to reduce zinc accumulation. Disruption of both systems also exerted pleiotropic effects on the homeostasis of other -block elements. Zinc limitation perturbed cell morphology and compromised resistance to stressors, such as salt and oxidative stress. The mutant strain lacking both systems showed significantly impaired virulence in acute lung infection models, highlighting the necessity of zinc acquisition in the virulence and pathogenicity of . PubMed: 38249299DOI: 10.3389/fcimb.2023.1322973 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.59 Å) |
Structure validation
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