8SVC

Crystal Structure of SBP from Klebsiella pneumoniae

8SVC の概要

• エントリーDOI: 10.2210/pdb8svc/pdb
• 分子名称: Metal ABC transporter substrate-binding protein, SULFATE ION, ZINC ION, ... (4 entities in total)
• 機能のキーワード: periplasmic solute-binding protein, metal binding protein
• 由来する生物種: Klebsiella pneumoniae
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34228.94

構造登録者

Giles, M.W.,Cole, G.B.,Ng, D.,McDevitt, C.A.,Moraes, T.F. (登録日: 2023-05-16, 公開日: 2024-01-24, 最終更新日: 2024-05-01)

主引用文献

Maunders, E.A.,Giles, M.W.,Ganio, K.,Cunningham, B.A.,Bennett-Wood, V.,Cole, G.B.,Ng, D.,Lai, C.C.,Neville, S.L.,Moraes, T.F.,McDevitt, C.A.,Tan, A.
Zinc acquisition and its contribution to Klebsiella pneumoniae virulence.
Front Cell Infect Microbiol, 13:1322973-1322973, 2023

PubMed Abstract: is a World Health Organization priority pathogen and a significant clinical concern for infections of the respiratory and urinary tracts due to widespread and increasing resistance to antimicrobials. In the absence of a vaccine, there is an urgent need to identify novel targets for therapeutic development. Bacterial pathogens, including , require the -block metal ion zinc as an essential micronutrient, which serves as a cofactor for ~6% of the proteome. During infection, zinc acquisition necessitates the use of high affinity uptake systems to overcome niche-specific zinc limitation and host-mediated nutritional immunity. Here, we report the identification of ZnuCBA and ZniCBA, two ATP-binding cassette permeases that are highly conserved in species and contribute to AJ218 zinc homeostasis, and the high-resolution structure of the zinc-recruiting solute-binding protein ZniA. The Znu and Zni permeases appear functionally redundant with abrogation of both systems required to reduce zinc accumulation. Disruption of both systems also exerted pleiotropic effects on the homeostasis of other -block elements. Zinc limitation perturbed cell morphology and compromised resistance to stressors, such as salt and oxidative stress. The mutant strain lacking both systems showed significantly impaired virulence in acute lung infection models, highlighting the necessity of zinc acquisition in the virulence and pathogenicity of .

PubMed: 38249299
DOI: 10.3389/fcimb.2023.1322973

実験手法

X-RAY DIFFRACTION (1.59 Å)