8SGP

human liver mitochondrial Medium-chain specific acyl-CoA dehydrogenase

8SGP の概要

• エントリーDOI: 10.2210/pdb8sgp/pdb
• EMDBエントリー: 40463
• 分子名称: Medium-chain specific acyl-CoA dehydrogenase, mitochondrial, FLAVIN-ADENINE DINUCLEOTIDE (2 entities in total)
• 機能のキーワード: human, liver, mitochondrial, medium-chain specific acyl-coa dehydrogenase, oxidoreductase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 189735.29

構造登録者

Zhang, Z. (登録日: 2023-04-12, 公開日: 2024-02-21, 最終更新日: 2025-05-28)

主引用文献

Zhang, Z.,Tringides, M.L.,Morgan, C.E.,Miyagi, M.,Mears, J.A.,Hoppel, C.L.,Yu, E.W.
High-Resolution Structural Proteomics of Mitochondria Using the 'Build and Retrieve' Methodology.
Mol.Cell Proteomics, 22:100666-100666, 2023

PubMed Abstract: The application of integrated systems biology to the field of structural biology is a promising new direction, although it is still in the infant stages of development. Here we report the use of single particle cryo-EM to identify multiple proteins from three enriched heterogeneous fractions prepared from human liver mitochondrial lysate. We simultaneously identify and solve high-resolution structures of nine essential mitochondrial enzymes with key metabolic functions, including fatty acid catabolism, reactive oxidative species clearance, and amino acid metabolism. Our methodology also identified multiple distinct members of the acyl-CoA dehydrogenase family. This work highlights the potential of cryo-EM to explore tissue proteomics at the atomic level.

PubMed: 37839702
DOI: 10.1016/j.mcpro.2023.100666

実験手法

ELECTRON MICROSCOPY (2.69 Å)