8SDZ

Structure of rat organic anion transporter 1 (OAT1) in complex with probenecid

Summary for 8SDZ

• Entry DOI: 10.2210/pdb8sdz/pdb
• EMDB information: 40355
• Descriptor: Solute carrier family 22 member 6, 4-(dipropylsulfamoyl)benzoic acid (3 entities in total)
• Functional Keywords: organic anion transporter, oat, slc22, drug transporter, probenecid, transport protein
• Biological source: Rattus norvegicus (Norway rat)
• Total number of polymer chains: 1
• Total formula weight: 61102.19

Authors

Dou, T.,Jiang, J. (deposition date: 2023-04-07, release date: 2023-10-18, Last modification date: 2024-11-13)

Primary citation

Dou, T.,Lian, T.,Shu, S.,He, Y.,Jiang, J.
The substrate and inhibitor binding mechanism of polyspecific transporter OAT1 revealed by high-resolution cryo-EM.
Nat.Struct.Mol.Biol., 30:1794-1805, 2023

PubMed Abstract: Organic anion transporters (OATs) of the SLC22 family have crucial roles in the transport of organic anions, including metabolites and therapeutic drugs, and in transporter-mediated drug-drug interactions. In the kidneys, OATs facilitate the elimination of metabolic waste products and xenobiotics. However, their transport activities can lead to the accumulation of certain toxic compounds within cells, causing kidney damage. Moreover, OATs are important drug targets, because their inhibition modulates the elimination or retention of substrates linked to diseases. Despite extensive research on OATs, the molecular basis of their substrate and inhibitor binding remains poorly understood. Here we report the cryo-EM structures of rat OAT1 (also known as SLC22A6) and its complexes with para-aminohippuric acid and probenecid at 2.1, 2.8 and 2.9 Å resolution, respectively. Our findings reveal a highly conserved substrate binding mechanism for SLC22 transporters, wherein four aromatic residues form a cage to accommodate the polyspecific binding of diverse compounds.

PubMed: 37845412
DOI: 10.1038/s41594-023-01123-3

Experimental method

ELECTRON MICROSCOPY (2.86 Å)