8SDU

Structure of rat organic anion transporter 1 (OAT1)

8SDU の概要

• エントリーDOI: 10.2210/pdb8sdu/pdb
• EMDBエントリー: 40352
• 分子名称: Solute carrier family 22 member 6 (2 entities in total)
• 機能のキーワード: organic anion transporter, oat, slc22, drug transporter, transport protein
• 由来する生物種: Rattus norvegicus (Norway rat)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 60816.84

構造登録者

Dou, T.,Jiang, J. (登録日: 2023-04-07, 公開日: 2023-10-18, 最終更新日: 2024-10-16)

主引用文献

Dou, T.,Lian, T.,Shu, S.,He, Y.,Jiang, J.
The substrate and inhibitor binding mechanism of polyspecific transporter OAT1 revealed by high-resolution cryo-EM.
Nat.Struct.Mol.Biol., 30:1794-1805, 2023

PubMed Abstract: Organic anion transporters (OATs) of the SLC22 family have crucial roles in the transport of organic anions, including metabolites and therapeutic drugs, and in transporter-mediated drug-drug interactions. In the kidneys, OATs facilitate the elimination of metabolic waste products and xenobiotics. However, their transport activities can lead to the accumulation of certain toxic compounds within cells, causing kidney damage. Moreover, OATs are important drug targets, because their inhibition modulates the elimination or retention of substrates linked to diseases. Despite extensive research on OATs, the molecular basis of their substrate and inhibitor binding remains poorly understood. Here we report the cryo-EM structures of rat OAT1 (also known as SLC22A6) and its complexes with para-aminohippuric acid and probenecid at 2.1, 2.8 and 2.9 Å resolution, respectively. Our findings reveal a highly conserved substrate binding mechanism for SLC22 transporters, wherein four aromatic residues form a cage to accommodate the polyspecific binding of diverse compounds.

PubMed: 37845412
DOI: 10.1038/s41594-023-01123-3

実験手法

ELECTRON MICROSCOPY (2.05 Å)