8SBE

Structure of the rat vesicular glutamate transporter 2 determined by single-particle Cryo-EM

「6V4D」から置き換えられました

8SBE の概要

• エントリーDOI: 10.2210/pdb8sbe/pdb
• EMDBエントリー: 21040
• 分子名称: Vesicular glutamate transporter 2 (1 entity in total)
• 機能のキーワード: glutamate transport, synaptic vesicle, major facilitator superfamily, excitatory neurotransmission, membrane protein
• 由来する生物種: Rattus norvegicus (Norway rat)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 58893.01

構造登録者

Li, F.,Finer-Moore, J.,Eriksen, J.,Cheng, Y.,Edwards, R.,Stroud, R. (登録日: 2023-04-03, 公開日: 2023-05-03, 最終更新日: 2024-06-19)

主引用文献

Li, F.,Eriksen, J.,Finer-Moore, J.,Chang, R.,Nguyen, P.,Bowen, A.,Myasnikov, A.,Yu, Z.,Bulkley, D.,Cheng, Y.,Edwards, R.H.,Stroud, R.M.
Ion transport and regulation in a synaptic vesicle glutamate transporter.
Science, 368:893-897, 2020

PubMed Abstract: Synaptic vesicles accumulate neurotransmitters, enabling the quantal release by exocytosis that underlies synaptic transmission. Specific neurotransmitter transporters are responsible for this activity and therefore are essential for brain function. The vesicular glutamate transporters (VGLUTs) concentrate the principal excitatory neurotransmitter glutamate into synaptic vesicles, driven by membrane potential. However, the mechanism by which they do so remains poorly understood owing to a lack of structural information. We report the cryo-electron microscopy structure of rat VGLUT2 at 3.8-angstrom resolution and propose structure-based mechanisms for substrate recognition and allosteric activation by low pH and chloride. A potential permeation pathway for chloride intersects with the glutamate binding site. These results demonstrate how the activity of VGLUTs can be coordinated with large shifts in proton and chloride concentrations during the synaptic vesicle cycle to ensure normal synaptic transmission.

PubMed: 32439795
DOI: 10.1126/science.aba9202

実験手法

ELECTRON MICROSCOPY (3.8 Å)