8S6H

Cryo-EM Structure of the R388 plasmid conjugative pilus reveals a helical polymer characterised by an unusual pilin/phospholipid binary complex

これはPDB形式変換不可エントリーです。

8S6H の概要

• エントリーDOI: 10.2210/pdb8s6h/pdb
• 関連するPDBエントリー: 5LEG 7JSV 8CW4 8EXH
• EMDBエントリー: 19758
• 分子名称: TrwL protein, 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE (2 entities in total)
• 機能のキーワード: conjugal transfer protein, virb2, conjugative pilus, type-4 secretion system fibre, conduit for horizontal gene transfer, anti-microbial resistance, protein fibril
• 由来する生物種: Escherichia coli HB101
• タンパク質・核酸の鎖数: 69
• 化学式量合計: 560259.44

構造登録者

Vadakkepat, A.K.,Waksman, G.,Redzej, A. (登録日: 2024-02-27, 公開日: 2024-07-24, 最終更新日: 2024-09-18)

主引用文献

Vadakkepat, A.K.,Xue, S.,Redzej, A.,Smith, T.K.,Ho, B.T.,Waksman, G.
Cryo-EM structure of the R388 plasmid conjugative pilus reveals a helical polymer characterized by an unusual pilin/phospholipid binary complex.
Structure, 32:1335-, 2024

PubMed Abstract: Bacterial conjugation is a process by which DNA is transferred unidirectionally from a donor cell to a recipient cell. It is the main means by which antibiotic resistance genes spread among bacterial populations. It is crucially dependent upon the elaboration of an extracellular appendage, termed "pilus," by a large double-membrane-spanning secretion system termed conjugative "type IV secretion system." Here we present the structure of the conjugative pilus encoded by the R388 plasmid. We demonstrate that, as opposed to all conjugative pili produced so far for cryoelectron microscopy (cryo-EM) structure determination, the conjugative pilus encoded by the R388 plasmid is greatly stimulated by the presence of recipient cells. Comparison of its cryo-EM structure with existing conjugative pilus structures highlights a number of important differences between the R388 pilus structure and that of its homologs, the most prominent being the highly distinctive conformation of its bound lipid.

PubMed: 39002540
DOI: 10.1016/j.str.2024.06.009

実験手法

ELECTRON MICROSCOPY (3.39 Å)