8S2X

SSX structure of Arabidopsis thaliana Pdx1.3 grown in microfluidic droplets

8S2X の概要

• エントリーDOI: 10.2210/pdb8s2x/pdb
• 分子名称: Pyridoxal 5'-phosphate synthase subunit PDX1.3, CITRIC ACID, PHOSPHATE ION, ... (4 entities in total)
• 機能のキーワード: vitamin b6, lyase
• 由来する生物種: Arabidopsis thaliana (thale cress)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 125876.61

構造登録者

Stubbs, J.,Tews, I.,Maly, M. (登録日: 2024-02-19, 公開日: 2024-02-28, 最終更新日: 2024-03-20)

主引用文献

Stubbs, J.,Hornsey, T.,Hanrahan, N.,Esteban, L.B.,Bolton, R.,Maly, M.,Basu, S.,Orlans, J.,de Sanctis, D.,Shim, J.U.,Shaw Stewart, P.D.,Orville, A.M.,Tews, I.,West, J.
Droplet microfluidics for time-resolved serial crystallography.
Iucrj, 11:237-248, 2024

PubMed Abstract: Serial crystallography requires large numbers of microcrystals and robust strategies to rapidly apply substrates to initiate reactions in time-resolved studies. Here, we report the use of droplet miniaturization for the controlled production of uniform crystals, providing an avenue for controlled substrate addition and synchronous reaction initiation. The approach was evaluated using two enzymatic systems, yielding 3 µm crystals of lysozyme and 2 µm crystals of Pdx1, an Arabidopsis enzyme involved in vitamin B6 biosynthesis. A seeding strategy was used to overcome the improbability of Pdx1 nucleation occurring with diminishing droplet volumes. Convection within droplets was exploited for rapid crystal mixing with ligands. Mixing times of <2 ms were achieved. Droplet microfluidics for crystal size engineering and rapid micromixing can be utilized to advance time-resolved serial crystallography.

PubMed: 38446456
DOI: 10.1107/S2052252524001799

実験手法

X-RAY DIFFRACTION (2.5 Å)