8S2W
SSX structure of Arabidopsis thaliana Pdx1.3 grown in seeded batch conditions
Summary for 8S2W
| Entry DOI | 10.2210/pdb8s2w/pdb |
| Descriptor | Pyridoxal 5'-phosphate synthase subunit PDX1.3, PHOSPHATE ION (3 entities in total) |
| Functional Keywords | vitamin b6, lyase |
| Biological source | Arabidopsis thaliana (thale cress) |
| Total number of polymer chains | 4 |
| Total formula weight | 125108.11 |
| Authors | Stubbs, J.,Tews, I.,Maly, M. (deposition date: 2024-02-19, release date: 2024-02-28, Last modification date: 2024-03-20) |
| Primary citation | Stubbs, J.,Hornsey, T.,Hanrahan, N.,Esteban, L.B.,Bolton, R.,Maly, M.,Basu, S.,Orlans, J.,de Sanctis, D.,Shim, J.U.,Shaw Stewart, P.D.,Orville, A.M.,Tews, I.,West, J. Droplet microfluidics for time-resolved serial crystallography. Iucrj, 11:237-248, 2024 Cited by PubMed Abstract: Serial crystallography requires large numbers of microcrystals and robust strategies to rapidly apply substrates to initiate reactions in time-resolved studies. Here, we report the use of droplet miniaturization for the controlled production of uniform crystals, providing an avenue for controlled substrate addition and synchronous reaction initiation. The approach was evaluated using two enzymatic systems, yielding 3 µm crystals of lysozyme and 2 µm crystals of Pdx1, an Arabidopsis enzyme involved in vitamin B6 biosynthesis. A seeding strategy was used to overcome the improbability of Pdx1 nucleation occurring with diminishing droplet volumes. Convection within droplets was exploited for rapid crystal mixing with ligands. Mixing times of <2 ms were achieved. Droplet microfluidics for crystal size engineering and rapid micromixing can be utilized to advance time-resolved serial crystallography. PubMed: 38446456DOI: 10.1107/S2052252524001799 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.5 Å) |
Structure validation
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