8RWK

cryoEM structure of the central Ald4 filament determined by FilamentID

8RWK の概要

• エントリーDOI: 10.2210/pdb8rwk/pdb
• EMDBエントリー: 19549
• 分子名称: Potassium-activated aldehyde dehydrogenase, mitochondrial, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE (2 entities in total)
• 機能のキーワード: metabolic enzyme, filament, cryoem, cytosolic protein
• 由来する生物種: Saccharomyces cerevisiae SK1
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 230123.18

構造登録者

Hugener, J.,Xu, J.,Wettstein, R.,Ioannidi, L.,Velikov, D.,Wollweber, F.,Henggeler, A.,Matos, J.,Pilhofer, M. (登録日: 2024-02-05, 公開日: 2024-06-26, 最終更新日: 2024-07-03)

主引用文献

Hugener, J.,Xu, J.,Wettstein, R.,Ioannidi, L.,Velikov, D.,Wollweber, F.,Henggeler, A.,Matos, J.,Pilhofer, M.
FilamentID reveals the composition and function of metabolic enzyme polymers during gametogenesis.
Cell, 187:3303-, 2024

PubMed Abstract: Gamete formation and subsequent offspring development often involve extended phases of suspended cellular development or even dormancy. How cells adapt to recover and resume growth remains poorly understood. Here, we visualized budding yeast cells undergoing meiosis by cryo-electron tomography (cryoET) and discovered elaborate filamentous assemblies decorating the nucleus, cytoplasm, and mitochondria. To determine filament composition, we developed a "filament identification" (FilamentID) workflow that combines multiscale cryoET/cryo-electron microscopy (cryoEM) analyses of partially lysed cells or organelles. FilamentID identified the mitochondrial filaments as being composed of the conserved aldehyde dehydrogenase Ald4 and the nucleoplasmic/cytoplasmic filaments as consisting of acetyl-coenzyme A (CoA) synthetase Acs1. Structural characterization further revealed the mechanism underlying polymerization and enabled us to genetically perturb filament formation. Acs1 polymerization facilitates the recovery of chronologically aged spores and, more generally, the cell cycle re-entry of starved cells. FilamentID is broadly applicable to characterize filaments of unknown identity in diverse cellular contexts.

PubMed: 38906101
DOI: 10.1016/j.cell.2024.04.026

実験手法

ELECTRON MICROSCOPY (3.8 Å)