8RV3

Structure of the domain IV of the replication factor RctB from Vibrio cholerae

8RV3 の概要

• エントリーDOI: 10.2210/pdb8rv3/pdb
• 分子名称: RctB, GLYCEROL, IODIDE ION, ... (4 entities in total)
• 機能のキーワード: replication initiation factor, rctb, vibrio cholerae, dna-protein interactions, replication
• 由来する生物種: Vibrio cholerae
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 42580.55

構造登録者

Garcia-Pino, A.,Talavera Perez, A. (登録日: 2024-01-31, 公開日: 2025-02-12, 最終更新日: 2025-08-27)

主引用文献

Niault, T.,Talavera, A.,Le Cam, E.,Baconnais, S.,Skovgaard, O.,Fournes, F.,Wagner, L.,Tamman, H.,Thompson, A.,Echemendia-Blanco, D.,Guzzi, N.,Garcia-Pino, A.,Mazel, D.,Val, M.E.
Dynamic transitions of initiator binding coordinate the replication of the two chromosomes in Vibrio cholerae.
Nat Commun, 16:485-485, 2025

PubMed Abstract: The replication of the two chromosomes in the pathogenic bacterium Vibrio cholerae is coordinated by the binding of initiator protein RctB to a checkpoint sequence, crtS. Replication of crtS on the primary chromosome (Chr1) triggers replication of the secondary chromosome (Chr2), but the details are poorly understood. Here, we analyze RctB binding patterns in the V. cholerae genome across various cell cycle stages. We find that RctB primarily binds to sites inhibiting replication initiation at the Chr2 origin (ori2). This inhibitory effect is counteracted when crtS is replicated on Chr1, causing a shift in RctB binding to sites that activate replication at ori2. Structural analyzes indicate the formation of diverse oligomeric states of RctB, coupled to the allosteric effect of DNA, which determine ori2 accessibility. We propose a synchronization model where, upon replication, crtS locally destabilizes the RctB inhibition complex, releasing the Chr2 replication origin.

PubMed: 39779702
DOI: 10.1038/s41467-024-55598-9

実験手法

X-RAY DIFFRACTION (2.35 Å)