8RTC

Rap from bacteriophage Phi3T in presence of pheromone RGHTS

これはPDB形式変換不可エントリーです。

8RTC の概要

• エントリーDOI: 10.2210/pdb8rtc/pdb
• 分子名称: Rap3T, Pheromone RGHTS, Glycerol ethoxylate, ... (4 entities in total)
• 機能のキーワード: rap, aspartate phosphatase, phi3t, bacteriophage, signaling protein
• 由来する生物種: Bacillus phage phi3T; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 94713.78

構造登録者

Felipe-Ruiz, A.,Zamora-Caballero, S.,Marina, A. (登録日: 2024-01-25, 公開日: 2024-07-24, 最終更新日: 2024-08-28)

主引用文献

Felipe-Ruiz, A.,Zamora-Caballero, S.,Bendori, S.O.,Penades, J.R.,Eldar, A.,Marina, A.
Extracellular proteolysis of tandemly duplicated pheromone propeptides affords additional complexity to bacterial quorum sensing.
Plos Biol., 22:e3002744-e3002744, 2024

PubMed Abstract: Bacterial interactions are vital for adapting to changing environments, with quorum sensing (QS) systems playing a central role in coordinating behaviors through small signaling molecules. The RRNPPA family is the prevalent QS systems in Bacillota and mediating communication through secreted oligopeptides, which are processed into active pheromones by extracellular proteases. Notably, in several cases the propeptides show the presence of multiple putative pheromones within their sequences, which has been proposed as a mechanism to diversify peptide-receptor specificity and potentially facilitate new functions. However, neither the processes governing the maturation of propeptides containing multiple pheromones, nor their functional significance has been evaluated. Here, using 2 Rap systems from bacteriophages infecting Bacillus subtilis that exhibit different types of pheromone duplication in their propeptides, we investigate the maturation process and the molecular and functional activities of the produced pheromones. Our results reveal that distinct maturation processes generate multiple mature pheromones, which bind to receptors with varying affinities but produce identical structural and biological responses. These findings add additional layers in the complexity of QS communication and regulation, opening new possibilities for microbial social behaviors, highlighting the intricate nature of bacterial interactions and adaptation.

PubMed: 39137235
DOI: 10.1371/journal.pbio.3002744

実験手法

X-RAY DIFFRACTION (2.83 Å)