8RPG

Crystal structure of an alcohol oxidase from Streptomyces hiroshimensis

8RPG の概要

• エントリーDOI: 10.2210/pdb8rpg/pdb
• 分子名称: Alcohol oxidase, FLAVIN-ADENINE DINUCLEOTIDE, TRIETHYLENE GLYCOL, ... (5 entities in total)
• 機能のキーワード: alcohol oxidase, streptomyces hiroshimensis, oxidoreductase, flavoprotein
• 由来する生物種: Streptomyces hiroshimensis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 58217.86

構造登録者

Martinez-Julvez, M.,Ferreira, P.,Cinca-Fernando, P. (登録日: 2024-01-15, 公開日: 2024-11-13)

主引用文献

Cinca-Fernando, P.,Ascaso-Alegre, C.,Sevilla, E.,Martinez-Julvez, M.,Mangas-Sanchez, J.,Ferreira, P.
Discovery, characterization, and synthetic potential of two novel bacterial aryl-alcohol oxidases.
Appl.Microbiol.Biotechnol., 108:498-498, 2024

PubMed Abstract: The search for novel synthetic tools to prepare industrial chemicals in a safer and greener manner is a continuing challenge in synthetic chemistry. In this manuscript, we report the discovery, characterization, and synthetic potential of two novel aryl-alcohol oxidases from bacteria which are able to oxidize a variety of aliphatic and aromatic alcohols with efficiencies up to 4970 min mM. Both enzymes have shown a reasonable thermostability (thermal melting temperature values of 50.9 and 48.6 °C for ShAAO and SdAAO, respectively). Crystal structures revealed an unusual wide-open entrance to the active-site pockets compared to that previously described for traditional fungal aryl-alcohol oxidases, which could be associated with differences observed in substrate scope, catalytic efficiency, and other functional properties. Preparative-scale reactions and the ability to operate at high substrate loadings also demonstrate the potential of these enzymes in synthetic chemistry with total turnover numbers > 38000. Moreover, their availability as soluble and active recombinant proteins enabled their use as cell-free extracts which further highlights their potential for the large-scale production of carbonyl compounds. KEY POINTS: • Identification and characterization of two novel bacterial aryl-alcohol oxidases • Crystal structures reveal wide-open active-site pockets, impacting substrate scope • Total turnover numbers and cell-free extracts demonstrate the synthetic potential.

PubMed: 39470785
DOI: 10.1007/s00253-024-13314-z

実験手法

X-RAY DIFFRACTION (2 Å)