8RIU

Crystal structure of the F420-reducing carbon monoxide dehydrogenase component from the ethanotroph Candidatus Ethanoperedens thermophilum

Summary for 8RIU

• Entry DOI: 10.2210/pdb8riu/pdb
• Descriptor: Acetyl-CoA decarbonylase/synthase complex subunit alpha, 2-{2-[2-(2-{2-[2-(2-ETHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL, CHLORIDE ION, ... (12 entities in total)
• Functional Keywords: carbon monoxide dehydrogenase, f420-reductase, acetyl-coenzyme a synthase-decarbonylase complex, anaerobic oxidoreductase, co2-releasing enzyme, c-cluster, co, co2, gas channelling., oxidoreductase
• Biological source: Candidatus Methanoperedenaceae archaeon GB50; More
• Total number of polymer chains: 6
• Total formula weight: 304171.02

Authors

Lemaire, O.N.,Wagner, T. (deposition date: 2023-12-19, release date: 2024-10-02, Last modification date: 2025-04-16)

Primary citation

Lemaire, O.N.,Wegener, G.,Wagner, T.
Ethane-oxidising archaea couple CO 2 generation to F 420 reduction.
Nat Commun, 15:9065-9065, 2024

PubMed Abstract: The anaerobic oxidation of alkanes is a microbial process that mitigates the flux of hydrocarbon seeps into the oceans. In marine archaea, the process depends on sulphate-reducing bacterial partners to exhaust electrons, and it is generally assumed that the archaeal CO-forming enzymes (CO dehydrogenase and formylmethanofuran dehydrogenase) are coupled to ferredoxin reduction. Here, we study the molecular basis of the CO-generating steps of anaerobic ethane oxidation by characterising native enzymes of the thermophile Candidatus Ethanoperedens thermophilum obtained from microbial enrichment. We perform biochemical assays and solve crystal structures of the CO dehydrogenase and formylmethanofuran dehydrogenase complexes, showing that both enzymes deliver electrons to the F cofactor. Both multi-metalloenzyme harbour electronic bridges connecting CO and formylmethanofuran oxidation centres to a bound flavin-dependent F reductase. Accordingly, both systems exhibit robust coupled F-reductase activities, which are not detected in the cell extract of related methanogens and anaerobic methane oxidisers. Based on the crystal structures, enzymatic activities, and metagenome mining, we propose a model in which the catabolic oxidising steps would wire electron delivery to F in this organism. Via this specific adaptation, the indirect electron transfer from reduced F to the sulphate-reducing partner would fuel energy conservation and represent the driving force of ethanotrophy.

PubMed: 39433727
DOI: 10.1038/s41467-024-53338-7

Experimental method

X-RAY DIFFRACTION (1.89 Å)