8RIT

Dimeric mutant S103D of the BTB domain of ZBTB8A from Xenopus laevis

8RIT の概要

• エントリーDOI: 10.2210/pdb8rit/pdb
• 分子名称: Zinc finger and BTB domain-containing protein 8A.1-A (1 entity in total)
• 機能のキーワード: transcription regulation, btb domain, polymerisation, transcription
• 由来する生物種: Xenopus laevis (African clawed frog)
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 105707.81

構造登録者

Coste, F.,Suskiewicz, M.J. (登録日: 2023-12-19, 公開日: 2024-06-05, 最終更新日: 2024-12-18)

主引用文献

Mance, L.,Bigot, N.,Zhamungui Sanchez, E.,Coste, F.,Martin-Gonzalez, N.,Zentout, S.,Biliskov, M.,Pukalo, Z.,Mishra, A.,Chapuis, C.,Arteni, A.A.,Lateur, A.,Goffinont, S.,Gaudon, V.,Talhaoui, I.,Casuso, I.,Beaufour, M.,Garnier, N.,Artzner, F.,Cadene, M.,Huet, S.,Castaing, B.,Suskiewicz, M.J.
Dynamic BTB-domain filaments promote clustering of ZBTB proteins.
Mol.Cell, 84:2490-2510.e9, 2024

PubMed Abstract: The formation of dynamic protein filaments contributes to various biological functions by clustering individual molecules together and enhancing their binding to ligands. We report such a propensity for the BTB domains of certain proteins from the ZBTB family, a large eukaryotic transcription factor family implicated in differentiation and cancer. Working with Xenopus laevis and human proteins, we solved the crystal structures of filaments formed by dimers of the BTB domains of ZBTB8A and ZBTB18 and demonstrated concentration-dependent higher-order assemblies of these dimers in solution. In cells, the BTB-domain filamentation supports clustering of full-length human ZBTB8A and ZBTB18 into dynamic nuclear foci and contributes to the ZBTB18-mediated repression of a reporter gene. The BTB domains of up to 21 human ZBTB family members and two related proteins, NACC1 and NACC2, are predicted to behave in a similar manner. Our results suggest that filamentation is a more common feature of transcription factors than is currently appreciated.

PubMed: 38996459
DOI: 10.1016/j.molcel.2024.05.029

実験手法

X-RAY DIFFRACTION (3.75 Å)