8RIN

Crystal structure of EccC5 DUF domain of Mycobacterium tuberculosis

8RIN の概要

• エントリーDOI: 10.2210/pdb8rin/pdb
• 分子名称: ESX-5 secretion system protein EccC5 (2 entities in total)
• 機能のキーワード: type vii secretion systems, duf, esx5, unknown function
• 由来する生物種: Mycobacterium tuberculosis H37Rv; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 65361.55

構造登録者

Ceballos-Zuniga, F.,Perez-Dorado, I. (登録日: 2023-12-19, 公開日: 2024-11-27)

主引用文献

Ceballos-Zuniga, F.,Menendez, M.,Perez-Dorado, I.
New insights into the domain of unknown function (DUF) of EccC 5 , the pivotal ATPase providing the secretion driving force to the ESX-5 secretion system.
Acta Crystallogr D Struct Biol, 80:397-409, 2024

PubMed Abstract: Type VII secretion (T7S) systems, also referred to as ESAT-6 secretion (ESX) systems, are molecular machines that have gained great attention due to their implications in cell homeostasis and in host-pathogen interactions in mycobacteria. The latter include important human pathogens such as Mycobacterium tuberculosis (Mtb), the etiological cause of human tuberculosis, which constitutes a pandemic accounting for more than one million deaths every year. The ESX-5 system is exclusively found in slow-growing pathogenic mycobacteria, where it mediates the secretion of a large family of virulence factors: the PE and PPE proteins. The secretion driving force is provided by EccC, a multidomain ATPase that operates using four globular cytosolic domains: an N-terminal domain of unknown function (EccC) and three FtsK/SpoIIIE ATPase domains. Recent structural and functional studies of ESX-3 and ESX-5 systems have revealed EccC to be an ATPase-like fold domain with potential ATPase activity, the functionality of which is essential for secretion. Here, the crystal structure of the MtbEccC domain is reported at 2.05 Å resolution, which reveals a nucleotide-free structure with degenerated cis-acting and trans-acting elements involved in ATP binding and hydrolysis. This crystallographic study, together with a biophysical assessment of the interaction of MtbEccC with ATP/Mg, supports the absence of ATPase activity proposed for this domain. It is shown that this degeneration is also present in DUF domains from other ESX and ESX-like systems, which are likely to exhibit poor or null ATPase activity. Moreover, based on an in silico model of the N-terminal region of MtbEccC, it is hypothesized that MtbEccC is a degenerated ATPase domain that may have retained the ability to hexamerize. These observations draw attention to DUF domains as structural elements with potential implications in the opening and closure of the membrane pore during the secretion process via their involvement in inter-protomer interactions.

PubMed: 38805245
DOI: 10.1107/S2059798324004248

実験手法

X-RAY DIFFRACTION (2.05 Å)