8RHZ

Structure of CUL9-RBX1 ubiquitin E3 ligase complex in unneddylated conformation - symmetry expanded unneddylated dimer

8RHZ の概要

• エントリーDOI: 10.2210/pdb8rhz/pdb
• 関連するPDBエントリー: 8Q7E 8Q7H
• EMDBエントリー: 18216 18217 18218 18220 18221 18222 18223 19179
• 分子名称: Cullin-9, E3 ubiquitin-protein ligase RBX1, ZINC ION (3 entities in total)
• 機能のキーワード: cullin-ring rbr e3 ligase, ligase
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 588344.53

構造登録者

Hopf, L.V.M.,Horn-Ghetko, D.,Prabu, J.R.,Schulman, B.A. (登録日: 2023-12-17, 公開日: 2024-04-17, 最終更新日: 2024-07-31)

主引用文献

Horn-Ghetko, D.,Hopf, L.V.M.,Tripathi-Giesgen, I.,Du, J.,Kostrhon, S.,Vu, D.T.,Beier, V.,Steigenberger, B.,Prabu, J.R.,Stier, L.,Bruss, E.M.,Mann, M.,Xiong, Y.,Schulman, B.A.
Noncanonical assembly, neddylation and chimeric cullin-RING/RBR ubiquitylation by the 1.8 MDa CUL9 E3 ligase complex.
Nat.Struct.Mol.Biol., 31:1083-1094, 2024

PubMed Abstract: Ubiquitin ligation is typically executed by hallmark E3 catalytic domains. Two such domains, 'cullin-RING' and 'RBR', are individually found in several hundred human E3 ligases, and collaborate with E2 enzymes to catalyze ubiquitylation. However, the vertebrate-specific CUL9 complex with RBX1 (also called ROC1), of interest due to its tumor suppressive interaction with TP53, uniquely encompasses both cullin-RING and RBR domains. Here, cryo-EM, biochemistry and cellular assays elucidate a 1.8-MDa hexameric human CUL9-RBX1 assembly. Within one dimeric subcomplex, an E2-bound RBR domain is activated by neddylation of its own cullin domain and positioning from the adjacent CUL9-RBX1 in trans. Our data show CUL9 as unique among RBX1-bound cullins in dependence on the metazoan-specific UBE2F neddylation enzyme, while the RBR domain protects it from deneddylation. Substrates are recruited to various upstream domains, while ubiquitylation relies on both CUL9's neddylated cullin and RBR domains achieving self-assembled and chimeric cullin-RING/RBR E3 ligase activity.

PubMed: 38605244
DOI: 10.1038/s41594-024-01257-y

実験手法

ELECTRON MICROSCOPY (3.37 Å)