8REY

Cuniculiplasma divulgatum filament

これはPDB形式変換不可エントリーです。

8REY の概要

• エントリーDOI: 10.2210/pdb8rey/pdb
• EMDBエントリー: 19112
• 分子名称: Flagellin-like protein, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-3)-[D-glycero-beta-D-galacto-heptopyranose-(1-2)]6-deoxy-6-sulfo-beta-D-galacto-heptopyranose-(1-4)-beta-D-glucopyranose-(1-3)-beta-D-galactopyranose (2 entities in total)
• 機能のキーワード: cell surface appendage, n-glycosylation, protein fibril
• 由来する生物種: Cuniculiplasma divulgatum
• タンパク質・核酸の鎖数: 36
• 化学式量合計: 575939.12

構造登録者

Isupov, M.N.,Gaines, M.,Daum, B.,McLaren, M. (登録日: 2023-12-12, 公開日: 2025-01-01, 最終更新日: 2025-10-01)

主引用文献

Gaines, M.C.,Isupov, M.N.,McLaren, M.,Haque, R.U.,Recalde, A.,Bargiela, R.,Gold, V.A.M.,Albers, S.V.,Golyshin, P.N.,Golyshina, O.V.,Daum, B.
Unusual cell surfaces, pili, and archaella of Thermoplasmatales archaea.
Isme J, 19:-, 2025

PubMed Abstract: Archaea of the order Thermoplasmatales push the boundaries of our current knowledge of prokaryotic life. They show distinct cellular plasticity, heterogenous cell morphologies, and lack a paracrystalline S-layer. As the S-layer has previously been implicated in acting as a stator scaffold for filaments driving cellular propulsion, particularly archaella, we asked whether the absence of an S-layer precludes the formation of functional archaella or pili in Thermoplasmatales. Using cryoEM, we investigated the two Thermoplasmatales species Cuniculiplasma divulgatum and Oxyplasma meridianum. We found that these species indeed generate pili and archaella and that the latter likely function in cellular propulsion. Whereas C. divulgatum produces pili with terminal hooks using a unique assembly machinery, O. meridianum generates unusually wide, "barbed" archaella with a high degree of glycosylation. Our results show that for the generation of functional archaella and pili, a canonical S-layer is not necessary.

PubMed: 40801261
DOI: 10.1093/ismejo/wraf176

実験手法

ELECTRON MICROSCOPY (2.61 Å)