8RCQ

Structural flexibility of Nucleoprotein of the Toscana virus in the presence of a nanobody.

「8BPK」から置き換えられました

8RCQ の概要

• エントリーDOI: 10.2210/pdb8rcq/pdb
• 分子名称: Nucleoprotein, VHH (2 entities in total)
• 機能のキーワード: toscana virus, nucleoprotein, saxs, vhh, nanobody, drugs, viral protein
• 由来する生物種: Toscana virus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 42614.51

構造登録者

Papageorgiou, N.,Ferron, F.,Coutard, B.,Lichiere, J.,Baklouti, A. (登録日: 2023-12-06, 公開日: 2024-01-31, 最終更新日: 2024-02-14)

主引用文献

Papageorgiou, N.,Baklouti, A.,Lichiere, J.,Desmyter, A.,Canard, B.,Coutard, B.,Ferron, F.
Structural flexibility of Toscana virus nucleoprotein in the presence of a single-chain camelid antibody.
Acta Crystallogr D Struct Biol, 80:113-122, 2024

PubMed Abstract: Phenuiviridae nucleoprotein is the main structural and functional component of the viral cycle, protecting the viral RNA and mediating the essential replication/transcription processes. The nucleoprotein (N) binds the RNA using its globular core and polymerizes through the N-terminus, which is presented as a highly flexible arm, as demonstrated in this article. The nucleoprotein exists in an `open' or a `closed' conformation. In the case of the closed conformation the flexible N-terminal arm folds over the RNA-binding cleft, preventing RNA adsorption. In the open conformation the arm is extended in such a way that both RNA adsorption and N polymerization are possible. In this article, single-crystal X-ray diffraction and small-angle X-ray scattering were used to study the N protein of Toscana virus complexed with a single-chain camelid antibody (VHH) and it is shown that in the presence of the antibody the nucleoprotein is unable to achieve a functional assembly to form a ribonucleoprotein complex.

PubMed: 38265877
DOI: 10.1107/S2059798324000196

実験手法

X-RAY DIFFRACTION (3.8 Å)