8RAS

Plastid-encoded RNA polymerase transcription elongation complex

これはPDB形式変換不可エントリーです。

8RAS の概要

• エントリーDOI: 10.2210/pdb8ras/pdb
• EMDBエントリー: 19023
• 分子名称: DNA-directed RNA polymerase subunit alpha, PAP6, PAP7, ... (26 entities in total)
• 機能のキーワード: transcription, chloroplast, rna polymerase, photosynthesis, gene regulation
• 由来する生物種: Sinapis alba (white mustard); 詳細
• タンパク質・核酸の鎖数: 23
• 化学式量合計: 1277075.31

構造登録者

Webster, M.W.,Pramanick, I.,Vergara-Cruces, A. (登録日: 2023-12-01, 公開日: 2024-03-06, 最終更新日: 2025-12-10)

主引用文献

Vergara-Cruces, A.,Pramanick, I.,Pearce, D.,Vogirala, V.K.,Byrne, M.J.,Low, J.K.K.,Webster, M.W.
Structure of the plant plastid-encoded RNA polymerase.
Cell, 187:1145-1159.e21, 2024

PubMed Abstract: Chloroplast genes encoding photosynthesis-associated proteins are predominantly transcribed by the plastid-encoded RNA polymerase (PEP). PEP is a multi-subunit complex composed of plastid-encoded subunits similar to bacterial RNA polymerases (RNAPs) stably bound to a set of nuclear-encoded PEP-associated proteins (PAPs). PAPs are essential to PEP activity and chloroplast biogenesis, but their roles are poorly defined. Here, we present cryoelectron microscopy (cryo-EM) structures of native 21-subunit PEP and a PEP transcription elongation complex from white mustard (Sinapis alba). We identify that PAPs encase the core polymerase, forming extensive interactions that likely promote complex assembly and stability. During elongation, PAPs interact with DNA downstream of the transcription bubble and with the nascent mRNA. The models reveal details of the superoxide dismutase, lysine methyltransferase, thioredoxin, and amino acid ligase enzymes that are subunits of PEP. Collectively, these data provide a foundation for the mechanistic understanding of chloroplast transcription and its role in plant growth and adaptation.

PubMed: 38428394
DOI: 10.1016/j.cell.2024.01.036

実験手法

ELECTRON MICROSCOPY (2.62 Å)