8R3O

Transketolase from Haemophilus influenzae in complex with thiamin pyrophosphate

8R3O の概要

• エントリーDOI: 10.2210/pdb8r3o/pdb
• 分子名称: Transketolase, THIAMINE DIPHOSPHATE, 1,2-ETHANEDIOL, ... (6 entities in total)
• 機能のキーワード: transketolase, pentose phosphate pathway, cytosolic protein, infectious organism
• 由来する生物種: Haemophilus influenzae
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 301399.11

構造登録者

Ballut, L.,Georges, R.N.,Aghajari, N.,Hecquet, L.,Charmantray, F.,Doumeche, B. (登録日: 2023-11-10, 公開日: 2024-05-22, 最終更新日: 2024-12-04)

主引用文献

Georges, R.N.,Ballut, L.,Aghajari, N.,Hecquet, L.,Charmantray, F.,Doumeche, B.
Biochemical, Bioinformatic, and Structural Comparisons of Transketolases and Position of Human Transketolase in the Enzyme Evolution.
Biochemistry, 63:1460-1473, 2024

PubMed Abstract: Transketolases (TKs) are key enzymes of the pentose phosphate pathway, regulating several other critical pathways in cells. Considering their metabolic importance, TKs are expected to be conserved throughout evolution. However, Tittmann et al. (, , 285(41): 31559-31570) demonstrated that TK (TK) possesses several structural and kinetic differences compared to bacterial TKs. Here, we study 14 TKs from pathogenic bacteria, fungi, and parasites and compare them with TK using biochemical, bioinformatic, and structural approaches. For this purpose, six new TK structures are solved by X-ray crystallography, including the TK of . All of these TKs have the same general fold as bacterial TKs. This comparative study shows that TK greatly differs from TKs from pathogens in terms of enzymatic activity, spatial positions of the active site, and monomer-monomer interface residues. An ubiquitous structural pattern is identified in all TKs as a six-residue histidyl crown around the TK cofactor (thiamine pyrophosphate), except for TK containing only five residues in the crown. Residue mapping of the monomer-monomer interface and the active site reveals that TK contains more unique residues than other TKs. From an evolutionary standpoint, TKs from animals (including ) and sp. belong to a distinct structural group from TKs of bacteria, plants, fungi, and parasites, mostly based on a different linker between domains, raising hypotheses regarding evolution and regulation.

PubMed: 38767928
DOI: 10.1021/acs.biochem.3c00714

実験手法

X-RAY DIFFRACTION (2.1 Å)