8QZU

XhpG hydrolase mutant S98A of Xenorhabdus hominickii

8QZU の概要

• エントリーDOI: 10.2210/pdb8qzu/pdb
• 分子名称: Carboxylesterase, ACETATE ION (3 entities in total)
• 機能のキーワード: alpha/beta hydrolase, post-nrps enzyme, tailoring enzyme, oxopyrrolizidinacetamide hydrolase, biosynthetic protein
• 由来する生物種: Xenorhabdus hominickii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29418.48

構造登録者

Calderari, A.,Gruez, A.,Weissman, K.J. (登録日: 2023-10-30, 公開日: 2024-11-06, 最終更新日: 2024-12-18)

主引用文献

Effert, J.,Westphalen, M.,Calderari, A.,Shi, Y.M.,Elamri, I.,Najah, S.,Grun, P.,Li, Y.,Gruez, A.,Weissman, K.J.,Bode, H.B.
Pyrrolizwilline, a Unique Bacterial Alkaloid Assembled by a Nonribosomal Peptide Synthetase and non-Enzymatic Dimerization.
Angew.Chem.Int.Ed.Engl., 63:e202411258-e202411258, 2024

PubMed Abstract: Pyrrolizidine alkaloids (PAs) are a structurally diverse group of heterocyclic specialized metabolites characterized by a core structure comprising a hexahydro-1H-pyrrolizine. PAs are synthesized through two main pathways. In plants, assembly occurs via a homospermidine synthase, and in bacteria, through combined action of a nonribosomal peptide synthetase and a Baeyer-Villiger monooxygenase. While the toxic properties of plant-derived PAs and their prevalence in animal and human foods have been extensively studied, the biological roles and biosynthesis of more complex bacterial PAs are not well understood. Here, we report the identification and characterization of a bacterial biosynthetic gene cluster from Xenorhabdus hominickii, xhpA-G, which is responsible for producing the PA pseudo-dimer pyrrolizwilline. Analysis of X. hominickii promoter exchange mutants together with heterologous expression of xhpA-G in E. coli, revealed a set of pathway intermediates, two of which were chemically synthesized, as well as multiple derivatives. This information was leveraged to propose a detailed biosynthetic pathway to pyrrolizwilline. Furthermore, we have characterized the hydrolase XhpG, the key enzyme in the conversion of the pathway intermediate pyrrolizixenamide to pyrrolizwilline, using X-ray crystallography and small-angle X-ray scattering (SAXS).

PubMed: 39428351
DOI: 10.1002/anie.202411258

実験手法

X-RAY DIFFRACTION (1.18 Å)