8QZ0

SWR1-hexasome-dimer complex

これはPDB形式変換不可エントリーです。

8QZ0 の概要

• エントリーDOI: 10.2210/pdb8qz0/pdb
• EMDBエントリー: 18769
• 分子名称: Histone H3, Actin-like protein ARP6, Vacuolar protein sorting-associated protein 71, ... (20 entities in total)
• 機能のキーワード: chromatin remodelling complex, hexasome-dimer complex, dna binding protein
• 由来する生物種: Saccharomyces cerevisiae S288C; 詳細
• タンパク質・核酸の鎖数: 22
• 化学式量合計: 893304.36

構造登録者

Jalal, A.S.B.,Wigley, D.B. (登録日: 2023-10-26, 公開日: 2024-10-02, 最終更新日: 2025-07-02)

主引用文献

Jalal, A.S.B.,Girvan, P.,Chua, E.Y.D.,Liu, L.,Wang, S.,McCormack, E.A.,Skehan, M.T.,Knight, C.L.,Rueda, D.S.,Wigley, D.B.
Stabilization of the hexasome intermediate during histone exchange by yeast SWR1 complex.
Mol.Cell, 84:3871-, 2024

PubMed Abstract: The yeast SWR1 complex catalyzes the exchange of histone H2A/H2B dimers in nucleosomes with Htz1/H2B dimers. We use cryoelectron microscopy to determine the structure of an enzyme-bound hexasome intermediate in the reaction pathway of histone exchange, in which an H2A/H2B dimer has been extracted from a nucleosome prior to the insertion of a dimer comprising Htz1/H2B. The structure reveals a key role for the Swc5 subunit in stabilizing the unwrapping of DNA from the histone core of the hexasome. By engineering a crosslink between an Htz1/H2B dimer and its chaperone protein Chz1, we show that this blocks histone exchange by SWR1 but allows the incoming chaperone-dimer complex to insert into the hexasome. We use this reagent to trap an SWR1/hexasome complex with an incoming Htz1/H2B dimer that shows how the reaction progresses to the next step. Taken together the structures reveal insights into the mechanism of histone exchange by SWR1 complex.

PubMed: 39226902
DOI: 10.1016/j.molcel.2024.08.015

実験手法

ELECTRON MICROSCOPY (3.8 Å)