8QUE

Structure of the Bacteriophage PhiKZ non-virion RNA Polymerase bound to DNA and RNA

8QUE の概要

• エントリーDOI: 10.2210/pdb8que/pdb
• EMDBエントリー: 12885 12886 18661
• 分子名称: PHIKZ055, ZINC ION, DNA-directed RNA polymerase, ... (10 entities in total)
• 機能のキーワード: phikz, nvrnap, rna, dna, transcription, rna binding protein
• 由来する生物種: Pseudomonas phage phiKZ; 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 391560.90

構造登録者

de Martin Garrido, N.,Yakunina, M.,Aylett, C.H.S. (登録日: 2023-10-16, 公開日: 2024-07-31, 最終更新日: 2024-08-07)

主引用文献

de Martin Garrido, N.,Chen, C.S.,Ramlaul, K.,Aylett, C.H.S.,Yakunina, M.
Structure of the Bacteriophage PhiKZ Non-virion RNA Polymerase Transcribing from its Promoter p119L.
J.Mol.Biol., 436:168713-168713, 2024

PubMed Abstract: Bacteriophage ΦKZ (phiKZ) is the founding member of a family of giant bacterial viruses. It has potential as a therapeutic as its host, Pseudomonas aeruginosa, kills tens of thousands of people worldwide each year. ΦKZ infection is independent of the host transcriptional apparatus; the virus forms a "nucleus", producing a proteinaceous barrier around the ΦKZ genome that excludes the host immune systems. It expresses its own non-canonical multi-subunit non-virion RNA polymerase (nvRNAP), which is imported into its "nucleus" to transcribe viral genes. The ΦKZ nvRNAP is formed by four polypeptides representing homologues of the eubacterial β/β' subunits, and a fifth that is likely to have evolved from an ancestral homologue to σ-factor. We have resolved the structure of the ΦKZ nvRNAP initiating transcription from its cognate promoter, p119L, including previously disordered domains and regions. Our results shed light on the similarities and differences between ΦKZ nvRNAP mechanisms of transcription and those of canonical eubacterial RNAPs and the related non-canonical nvRNAP of bacteriophage AR9.

PubMed: 39029888
DOI: 10.1016/j.jmb.2024.168713

実験手法

ELECTRON MICROSCOPY (3.3 Å)